Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRG3 / GPR97 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105608-100
Catalog Number: LS-G105608
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR97(ADGRG3) (NM_170776), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 97 | GPR97 | PGR26 | PB99
- Origin Species
- Human
- NCBI GenBank
- NM_170776
- SwissProt
- Q86Y34
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATPRGLGAL LLLLLLPTSG QEKPTEGPRN TCLGSNNMYD IFNLNDKALC FTKCRQSGSD61SCNVENLQRY WLNYEAHLMK EGLTQKVNTP FLKALVQNLS TNTAEDFYFS LEPSQVPRQV121MKDEDKPPDR VRLPKSLFRS LPGNRSVVRL AVTILDIGPG TLFKGPRLGL GDGSGVLNNR181LVGLSVGQMH VTKLAEPLEI VFSHQRPPPN MTLTCVFWDV TKGTTGDWSS EGCSTEVRPE241GTVCCCDHLT FFALLLRPTL DQSTVHILTR ISQAGCGVSM IFLAFTIILY AFLRLSRERF301KSEDAPKIHV ALGGSLFLLN LAFLVNVGSG SKGSDAACWA RGAVFHYFLL CAFTWMGLEA361FHLYLLAVRV FNTYFGHYFL KLSLVGWGLP ALMVIGTGSA NSYGLYTIRD RENRTSLELC421WFREGTTMYA LYITVHGYFL ITFLFGMVVL ALVVWKIFTL SRATVVKERG KNRKKVLTLL481GLSSLVGVTW GLAIFTPLGL STVYIFALFN SLQGVFICCW FTILYLPSQS TTVSSSTARL541DQAHSASQETRTRPLEQKLISEEDLAANDILDYKDDDDKV
549 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.