Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRF3 / GPR113 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126570-100
Catalog Number: LS-G126570
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR113(ADGRF3) (NM_001145168), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 113 | GPR113 | HGPCR37 | G protein-coupled receptor 113 | PGR23
- Origin Species
- Human
- NCBI GenBank
- AY140955
- SwissProt
- Q8IZF5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVCSAAPLLL LATTLPLLGS PVAQASQPVS ETGVRPREGL QRRQWGPLIG RDKAWNERID61RPFPACPIPL SSSFGRWPKG QTMWAQTSTL TLTEEELGQS QAGGESGSGQ LLDQENGAGE121SALVSVYVHL DFPDKTWPPE LSRTLTLPAA SASSSPRPLL TGLRLTTECN VNHKGNFYCA181CLSGYQWNTS ICLHYPPCQS LHNHQPCGCL VFSHPEPGYC QLLPPGSPVT CLPAVPGILN241LNSQLQMPGD TLSLTLHLSQ EATNLSWFLR HPGSPSPILL QPGTQVSVTS SHGQAALSVS301NMSHHWAGEY MSCFEAQGFK WNLYEVVRVP LKATDVARLP YQLSISCATS PGFQLSCCIP361STNLAYTAAW SPGEGSKASS FNESGSQCFV LAVQRCPMAD TTYACDLQSL GLAPLRVPIS421ITIIQDGDIT CPEDASVLTW NVTKAGHVAQ APCPESKRGI VRRLCGADGV WGPVHSSCTD481ARLLALFTRT KLLQAGQGSP AEEVPQILAQ LPGQAAEASS PSDLLTLLST MKYVAKVVAE541ARIQLDRRAL KNLLIATDKV LDMDTRSLWT LAQARKPWAG STLLLAVETL ACSLCPQDHP601FAFSLPNVLL QSQLFGPTFP ADYSISFPTR PPLQAQIPRH SLAPLVRNGT EISITSLVLR661KLDHLLPSNY GQGLGDSLYA TPGLVLVISI MAGDRAFSQG EVIMDFGNTD GSPHCVFWDH721SLFQGRGGWS KEGCQAQVAS ASPTAQCLCQ HLTAFSVLMS PHTVPEEPAL ALLTQVGLGA781SILALLVCLG VYWLVWRVVV RNKISYFRHA ALLNMVFCLL AADTCFLGAP FLSPGPRSPL841CLAAAFLCHF LYLATFFWML AQALVLAHQL LFVFHQLAKH RVLPLMVLLG YLCPLGLAGV901TLGLYLPQGQ YLREGECWLD GKGGALYTFV GPVLAIIGVN GLVLAMAMLK LLRPSLSEGP961PAEKRQALLG VIKALLILTP IFGLTWGLGL ATLLEEVSTV PHYIFTILNT LQGVFILLFG1021CLMDRKIQEA LRKRFCRAQA PSSTISLVSC CLQILSCASK SMSEGIPWPS SEDMGTARSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1079 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.