Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRF1 / GPR110 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105655-100
Catalog Number: LS-G105655
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR110(ADGRF1) (NM_153840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 110 | G-protein coupled receptor 110 | GPR110 | HGPCR36 | KPG_012 | PGR19
- Origin Species
- Human
- NCBI GenBank
- NM_025048
- SwissProt
- Q5T601
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVGVLWLIS FFTFTDGHGG FLGKNDGIKT KKELIVNKKK HLGPVEEYQL LLQVTYRDSK61EKRDLRNFLK LLKPPLLWSH GLIRIIRAKA TTDCNSLNGV LQCTCEDSYT WFPPSCLDPQ121NCYLHTAGAL PSCECHLNNL SQSVNFCERT KIWGTFKINE RFTNDLLNSS SAIYSKYANG181IEIQLKKAYE RIQGFESVQV TQFRNGSIVA GYEVVGSSSA SELLSAIEHV AEKAKTALHK241LFPLEDGSFR VFGKAQCNDI VFGFGSKDDE YTLPCSSGYR GNITAKCESS GWQVIRETCV301LSLLEELNKN FSMIVGNATE AAVSSFVQNL SVIIRQNPST TVGNLASVVS ILSNISSLSL361ASHFRVSNST MEDVISIADN ILNSASVTNW TVLLREEKYA SSRLLETLEN ISTLVPPTAL421PLNFSRKFID WKGIPVNKSQ LKRGYSYQIK MCPQNTSIPI RGRVLIGSDQ FQRSLPETII481SMASLTLGNI LPVSKNGNAQ VNGPVISTVI QNYSINEVFL FFSKIESNLS QPHCVFWDFS541HLQWNDAGCH LVNETQDIVT CQCTHLTSFS ILMSPFVPST IFPVVKWITY VGLGISIGSL601ILCLIIEALF WKQIKKSQTS HTRRICMVNI ALSLLIADVW FIVGATVDTT VNPSGVCTAA661VFFTHFFYLS LFFWMLMLGI LLAYRIILVF HHMAQHLMMA VGFCLGYGCP LIISVITIAV721TQPSNTYKRK DVCWLNWSNG SKPLLAFVVP ALAIVAVNFV VVLLVLTKLW RPTVGERLSR781DDKATIIRVG KSLLILTPLL GLTWGFGIGT IVDSQNLAWH VIFALLNAFQ GFFILCFGIL841LDSKLRQLLF NKLSALSSWK QTEKQNSSDL SAKPKFSKPF NPLQNKGHYA FSHTGDSSDN901IMLTQFVSNETRTRPLEQKLISEEDLAANDILDYKDDDDKV
910 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.