Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRD2 / GPR144 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128402-100
Catalog Number: LS-G128402
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR144(GPR144) (NM_001161808), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 144 | PGR24 | GPR144
- Origin Species
- Human
- NCBI GenBank
- XR_042073
- SwissProt
- Q7Z7M1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAPWGAGER WLHGAAVDRS GVSLGPPPTP QVNQGTLGPQ VAPVAAGEVV KTAGGVCKFS61GQRLSWWQAQ ESCEQQFGHL ALQPPDGVLA SRLRDPVWVG QREAPLRRPP QRRARTTAVL121VFDERTADRA ARLRSPLPEL AALTACTHVQ WDCASPDPAA LFSVAAPALP NALQLRAFAE181PGGVVRAALV VRGQHAPFLA AFRADGRWHH VCATWEQRGG RWALFSDGRR RAGARGLGAG241HPVPSGGILV LGQDQDSLGG GFSVRHALSG NLTDFHLWAR ALSPAQLHRA RACAPPSEGL301LFRWDPGALD VTPSLLPTVW VRLLCPVPSE ECPTWNPGPR SEGSELCLEP QPFLCCYRTE361PYRRLQDAQS WPGQDVISRV NALANDIVLL PDPLSEVHGA LSPAEASSFL GLLEHVLAME421MAPLGPAALL AVVRFLKRVV ALGAGDPELL LTGPWEQLSQ GVVSVASLVL EEQVADTWLS481LREVIGGPMA LVASVQRLAP LLSTSMTSER PRMRIQHRHA GLSGVTVIHS WFTSRVFQHT541LEGPDLEPQA PASSEEANRV QRFLSTQVGS AIISSEVWDV TGEVNVAMTF HLQHRAQSPL601FPPHPPSPYT GGAWATTGCS VAALYLDSTA CFCNHSTSFA ILLQIYEVQR GPEEESLLRT661LSFVGCGVSF CALTTTFLLF LVAGVPKSER TTVHKNLTFS LASAEGFLMT SEWAKANEVA721CVAVTVAMHF LFLVAFSWML VEGLLLWRKV VAVSMHPGPG MRLYHATGWG VPVGIVAVTL781AMLPHDYVAP GHCWLNVHTN AIWAFVGPVL FVLTANTCIL ARVVMITVSS ARRRARMLSP841QPCLQQQIWT QIWATVKPVL VLLPVLGLTW LAGILVHLSP AWAYAAVGLN SIQGLYIFLV901YAACNEEVRS ALQRMAEKKV AEVLRALGVW GGAAKEHSLP FSVLPLFLPP KPSTPRHPLK961APATRTRPLEQKLISEEDLAANDILDYKDDDDKV
963 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.