Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRA2 / GPR124 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G129178-100
Catalog Number: LS-G129178
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR124(ADGRA2) (NM_032777), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 124 | G-protein coupled receptor 124 | TEM5 | GPR124 | KIAA1531 | Tumor endothelial marker 5
- Origin Species
- Human
- NCBI GenBank
- AY181242
- SwissProt
- Q96PE1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 142.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAGGRRMRG APARLLLPLL PWLLLLLAPE ARGAPGCPLS IRSCKCSGER PKGLSGGVPG61PARRRVVCSG GDLPEPPEPG LLPNGTVTLL LSNNKITGLR NGSFLGLSLL EKLDLRNNII121STVQPGAFLG LGELKRLDLS NNRIGCLTSE TFQGLPRLLR LNISGNIFSS LQPGVFDELP181ALKVVDLGTE FLTCDCHLRW LLPWAQNRSL QLSEHTLCAY PSALHAQALG SLQEAQLCCE241GALELHTHHL IPSLRQVVFQ GDRLPFQCSA SYLGNDTRIR WYHNRAPVEG DEQAGILLAE301SLIHDCTFIT SELTLSHIGV WASGEWECTV SMAQGNASKK VEIVVLETSA SYCPAERVAN361NRGDFRWPRT LAGITAYQSC LQYPFTSVPL GGGAPGTRAS RRCDRAGRWE PGDYSHCLYT421NDITRVLYTF VLMPINASNA LTLAHQLRVY TAEAASFSDM MDVVYVAQMI QKFLGYVDQI481KELVEVMVDM ASNLMLVDEH LLWLAQREDK ACSRIVGALE RIGGAALSPH AQHISVNARN541VALEAYLIKP HSYVGLTCTA FQRREGGVPG TRPGSPGQNP PPEPEPPADQ QLRFRCTTGR601PNVSLSSFHI KNSVALASIQ LPPSLFSSLP AALAPPVPPD CTLQLLVFRN GRLFHSHSNT661SRPGAAGPGK RRGVATPVIF AGTSGCGVGN LTEPVAVSLR HWAEGAEPVA AWWSQEGPGE721AGGWTSEGCQ LRSSQPNVSA LHCQHLGNVA VLMELSAFPR EVGGAGAGLH PVVYPCTALL781LLCLFATIIT YILNHSSIRV SRKGWHMLLN LCFHIAMTSA VFAGGITLTN YQMVCQAVGI841TLHYSSLSTL LWMGVKARVL HKELTWRAPP PQEGDPALPT PSPMLRFYLI AGGIPLIICG901ITAAVNIHNY RDHSPYCWLV WRPSLGAFYI PVALILLITW IYFLCAGLRL RGPLAQNPKA961GNSRASLEAG EELRGSTRLR GSGPLLSDSG SLLATGSARV GTPGPPEDGD SLYSPGVQLG1021ALVTTHFLYL AMWACGALAV SQRWLPRVVC SCLYGVAASA LGLFVFTHHC ARRRDVRASW1081RACCPPASPA APHAPPRALP AAAEDGSPVF GEGPPSLKSS PSGSSGHPLA LGPCKLTNLQ1141LAQSQVCEAG AAAGGEGEPE PAGTRGNLAH RHPNNVHHGR RAHKSRAKGH RAGEACGKNR1201LKALRGGAAG ALELLSSESG SLHNSPTDSY LGSSRNSPGA GLQLEGEPML TPSEGSDTSA1261APLSEAGRAG QRRSASRDSL KGGGALEKES HRRSYPLNAA SLNGAPKGGK YDDVTLMGAE1321VASGGCMKTG LWKSETTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1338 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.