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ADCK5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADCK5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADCK5 antibodies →
$504.00each
Catalog Number: LS-G105144-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADCK5 (NM_174922), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADCK5
Origin Species
Human
NCBI GenBank
AK092773

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWRPVQLCHF HSALLHSRQK PWPSPAVFFR RNVRGLPPRF SSPTPLWRKV LSTAVVGAPL
61LLGARYVMAE AREKRRMRLV VDGMGRFGRS LKVGLQISLD YWWCTNVVLR GVEENSPGYL
121EVMSACHQRA ADALVAGAIS NGGLYVKLGQ GLCSFNHLLP PEYTRTLRVL EDRALKRGFQ
181EVDELFLEDF QALPHELFQE FDYQPIAAAS LAQVHRAKLH DGTSVAVKVQ YIDLRDRFDG
241DIHTLELLLR LVEVMHPSFG FSWVLQDLKG TLAQELDFEN EGRNAERCAR ELAHFPYVVV
301PRVHWDKSSK RVLTADFCAG CKVNDVEAIR SQGLAVHDIA EKLIKAFAEQ IFYTGFIHSD
361PHPGNVLVRK GPDGKAELVL LDHGLYQFLE EKDRAALCQL WRAIILRDDA AMRAHAAALG
421VQDYLLFAEM LMQRPVRLGQ LWGSHLLSRE EAAYMVDMAR ERFEAVMAVL RELPRPMLLV
481LRNINTVRAI NVALGAPVDR YFLMAKRAVR GWSRLAGATY RGVYGTSLLR HAKVVWEMLK
541FEVALRLETL AMRLTALLAR ALVHLSLVPP AEELYQYLET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

580 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.