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ADCK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADCK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADCK2 antibodies →
$725.00each
Catalog Number: LS-G102143-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADCK2 (NM_052853), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AARF | ADCK2
Origin Species
Human
NCBI GenBank
NM_052853
SwissProt
Q7Z695

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVAPWRVSVR VCLSHLRCFE LRQGLSLLRP SECPRDARLC WLLLGTLPKV VSLCGDVGEG
61APDVLSRRRV RCSGAAGAGP AESLPRAGPL GGVFLHLRLW LRAGALLVKF FPLLLLYPLT
121YLAPSVSTLW LHLLLKATET SGPTYIKLGQ WASTRRDLFS EAFCAQFSKL HVRVTPHPWT
181HTERFLRQAF GDDWGSILSF ENREPVGSGC VAQVYKAYAN TAFLETDSVQ RLGRASCLPP
241FSHTGAVGGL RELFGYLGNG RKPPENLADQ SFLERLLLPK ADLVGSNAGV SRAQVPGHQP
301EATNLISVAV KVLHPGLLAQ VHMDLLLMKI GSRVLGVLPG IKWLSLPEIV EEFEKLMVQQ
361IDLRYEAQNL EHFQVNFRNV KAVKFPTPLR PFVTREVLVE TYEESVPVSS YQQAGIPVDL
421KRKIARLGIN MLLKMIFVDN FVHADLHPGN ILVQGANGLS SSQEAQLQQA DICDTLVVAV
481PSSLCPLRLV LLDAGIVAEL QAPDLRNFRA VFMAVVMGQG QRVAELILHH ARASECRDVE
541GFKTEMAMLV TQARKNTITL EKLHVSSLLS SVFKLLMTHK VKLESNFASI VFAIMVLEGL
601GRSLDPKLDI LEAARPFLLT GPVCPP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.