Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADAP2 / CENTA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111754-100
Catalog Number: LS-G111754
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Centaurin alpha 2(ADAP2) (NM_018404), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADAP2 | Cent-b | Cnt-a2 | CENTA2 | Centaurin-alpha-2 | Centaurin | alpha 2 | Centaurin-alpha 2 protein | HSA272195 | ArfGAP with dual PH domains 2 | Centaurin beta
- Origin Species
- Human
- NCBI GenBank
- NM_018404
- SwissProt
- Q9NPF8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG VHRNFPDISR61VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP QANDCLVLKE QWIRAKYERR121EFMADGETIS LPGNREGFLW KRGRDNSQFL RRKFVLLARE GLLKYFTKEQ GKSPKAVISI181KDLNATFQTE KIGHPHGLQI TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF241PELPESELVP FLTRNYLKQG FMEKTGPKKE PFKKRWFALD CHERRLLYYK NPLDAFEQGQ301VFLGNKEQGY EAYEDLPKGI RGNRWKAGLT IVTPERRFVL TCPSEKEQQE WLESLRGVLS361SPLTPLNRLT ASTESGRSSRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
380 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.