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ADAMTSL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTSL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102709-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTSL3 (NM_207517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAMTS-like protein 3 | ADAMTSL-3 | ADAMTSL3 | ADAM-TSL3 | ADAMTS-like 3 | KIAA1233 | Punctin-2
Origin Species
Human
NCBI GenBank
AF237652
SwissProt
P82987

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
185.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASWTSPWWV LIGMVFMHSP LPQTTAEKSP GAYFLPEFAL SPQGSFLEDT TGEQFLTYRY
61DDQTSRNTRS DEDKDGNWDA WGDWSDCSRT CGGGASYSLR RCLTGRNCEG QNIRYKTCSN
121HDCPPDAEDF RAQQCSAYND VQYQGHYYEW LPRYNDPAAP CALKCHAQGQ NLVVELAPKV
181LDGTRCNTDS LDMCISGICQ AVGCDRQLGS NAKEDNCGVC AGDGSTCRLV RGQSKSHVSP
241EKREENVIAV PLGSRSVRIT VKGPVHLFIE SKTLQGSKGE HSFNSPGVFV VENTTVEFQR
301GSERQTFKIP GPLMADFIFK TRYTAAKDSV VQFFFYQPIS HQWRQTDFFP CTVTCGGGYQ
361LNSAECVDIR LKRVVPDHYC HYYPENVKPK PKLKECSMDP CPSSDGFKEI MPYDHFQPLP
421RWEHNPWTAC SVSCGGGIQR RSFVCVEESM HGEILQVEEW KCMYAPKPKV MQTCNLFDCP
481KWIAMEWSQC TVTCGRGLRY RVVLCINHRG EHVGGCNPQL KLHIKEECVI PIPCYKPKEK
541SPVEAKLPWL KQAQELEETR IATEEPTFIP EPWSACSTTC GPGVQVREVK CRVLLTFTQT
601ETELPEEECE GPKLPTERPC LLEACDESPA SRELDIPLPE DSETTYDWEY AGFTPCTATC
661LGGHQEAIAV CLHIQTQQTV NDSLCDMVHR PPAMSQACNT EPCPPRWHVG SWGPCSATCG
721VGIQTRDVYC LHPGETPAPP EECRDEKPHA LQACNQFDCP PGWHIEEWQQ CSRTCGGGTQ
781NRRVTCRQLL TDGSFLNLSD ELCQGPKASS HKSCARTDCP PHLAVGDWSK CSVSCGVGIQ
841RRKQVCQRLA AKGRRIPLSE MMCRDLPGLP LVRSCQMPEC SKIKSEMKTK LGEQGPQILS
901VQRVYIQTRE EKRINLTIGS RAYLLPNTSV IIKCPVRRFQ KSLIQWEKDG RCLQNSKRLG
961ITKSGSLKIH GLAAPDIGVY RCIAGSAQET VVLKLIGTDN RLIARPALRE PMREYPGMDH
1021SEANSLGVTW HKMRQMWNNK NDLYLDDDHI SNQPFLRALL GHCSNSAGST NSWELKNKQF
1081EAAVKQGAYS MDTAQFDELI RNMSQLMETG EVSDDLASQL IYQLVAELAK AQPTHMQWRG
1141IQEETPPAAQ LRGETGSVSQ SSHAKNSGKL TFKPKGPVLM RQSQPPSISF NKTINSRIGN
1201TVYITKRTEV INILCDLITP SEATYTWTKD GTLLQPSVKI ILDGTGKIQI QNPTRKEQGI
1261YECSVANHLG SDVESSSVLY AEAPVILSVE RNITKPEHNH LSVVVGGIVE AALGANVTIR
1321CPVKGVPQPN ITWLKRGGSL SGNVSLLFNG SLLLQNVSLE NEGTYVCIAT NALGKAVATS
1381VLHLLERRWP ESRIVFLQGH KKYILQATNT RTNSNDPTGE PPPQEPFWEP GNWSHCSATC
1441GHLGARIQRP QCVMANGQEV SEALCDHLQK PLAGFEPCNI RDCPARWFTS VWSQCSVSCG
1501EGYHSRQVTC KRTKANGTVQ VVSPRACAPK DRPLGRKPCF GHPCVQWEPG NRCPGRCMGR
1561AVRMQQRHTA CQHNSSDSNC DDRKRPTLRR NCTSGACDVC WHTGPWKPCT AACGRGFQSR
1621KVDCIHTRSC KPVAKRHCVQ KKKPISWRHC LGPSCDRDCT DTTHYCMFVK HLNLCSLDRY
1681KQRCCQSCQE G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1691 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.