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ADAMTSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108126-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTSL1 (NM_052866), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAMTSL1 | ADAM-TS related protein 1 | ADAMTSR1 | ADAMTS-like protein 1 | ADAMTSL-1 | C9orf94 | ADAMTS-like 1 | Punctin-1 | PUNCTIN
Origin Species
Human
NCBI GenBank
NM_052866
SwissProt
Q8N6G6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MECCRRATPG TLLLFLAFLL LSSRTARSEE DRDGLWDAWG PWSECSRTCG GGASYSLRRC
61LSSKSCEGRN IRYRTCSNVD CPPEAGDFRA QQCSAHNDVK HHGQFYEWLP VSNDPDNPCS
121LKCQAKGTTL VVELAPKVLD GTRCYTESLD MCISGLCQIV GCDHQLGSTV KEDNCGVCNG
181DGSTCRLVRG QYKSQLSATK SDDTVVAIPY GSRHIRLVLK GPDHLYLETK TLQGTKGENS
241LNSTGTFLVD NSSVDFQKFP DKEILRMAGP LTADFIVKIR NSGSADSTVQ FIFYQPIIHR
301WRETDFFPCS ATCGGGYQLT SAECYDLRSN RVVADQYCHY YPENIKPKPK LQECNLDPCP
361ASDGYKQIMP YDLYHPLPRW EATPWTACSS SCGGGIQSRA VSCVEEDIQG HVTSVEEWKC
421MYTPKMPIAQ PCNIFDCPKW LAQEWSPCTV TCGQGLRYRV VLCIDHRGMH TGGCSPKTKP
481HIKEECIVPT PCYKPKEKLP VEAKLPWFKQ AQELEEGAAV SEEPS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

525 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.