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ADAMTS8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTS8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAMTS8 antibodies →
$725.00each
Catalog Number: LS-G100963-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTS8 (NM_007037), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-TS 8 | ADAM-TS8 | ADAMTS-8 | ADAMTS8 | METH2
Origin Species
Human
NCBI GenBank
NM_007037
SwissProt
Q9UP79

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPAPAAPRW PPLLLLLLLL PLARGAPARP AAGGQASELV VPTRLPGSAG ELALHLSAFG
61KGFVLRLAPD DSFLAPDFKI ERLGGSGRAT GGERGLRGCF FSGTVNGEPE SLAAVSLCRG
121LSGSFLLDGE EFTIQPQGAG GSLAQPHRLQ RWGPAGARPL PRGPEWEVET GEGQRQERGD
181HQEDSEEESQ EEEAEGASEP PPPLGATSRT KRFVSEARFV ETLLVADASM AAFYGADLQN
241HILTLMSVAA RIYKHPSIKN SINLMVVKVL IVEDEKWGPE VSDNGGLTLR NFCNWQRRFN
301QPSDRHPEHY DTAILLTRQN FCGQEGLCDT LGVADIGTIC DPNKSCSVIE DEGLQAAHTL
361AHELGHVLSM PHDDSKPCTR LFGPMGKHHV MAPLFVHLNQ TLPWSPCSAM YLTELLDGGH
421GDCLLDAPAA ALPLPTGLPG RMALYQLDQQ CRQIFGPDFR HCPNTSAQDV CAQLWCHTDG
481AEPLCHTKNG SLPWADGTPC GPGHLCSEGS CLPEEEVERP KPVVDGGWAP WGPWGECSRT
541CGGGVQFSHR ECKDPEPQNG GRYCLGRRAK YQSCHTEECP PDGKSFREQQ CEKYNAYNYT
601DMDGNLLQWV PKYAGVSPRD RCKLFCRARG RSEFKVFEAK VIDGTLCGPE TLAICVRGQC
661VKAGCDHVVD SPRKLDKCGV CGGKGNSCRK VSGSLTPTNY GYNDIVTIPA GATNIDVKQR
721SHPGVQNDGN YLALKTADGQ YLLNGNLAIS AIEQDILVKG TILKYSGSIA TLERLQSFRP
781LPEPLTVQLL TVPGEVFPPK VKYTFFVPND VDFSMQSSKE RATTNIIQPL LHAQWVLGDW
841SECSSTCGAG WQRRTVECRD PSGQASATCN KALKPEDAKP CESQLCPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

888 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.