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ADAMTS19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTS19 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAMTS19 antibodies →
$725.00each
Catalog Number: LS-G107467-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTS19 (NM_133638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-TS 19 | ADAMTS-19 | ADAMTS19 | ADAM-TS19
Origin Species
Human
NCBI GenBank
NM_133638
SwissProt
Q8TE59

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLTHICCCC LLYQLGFLSN GIVSELQFAP DREEWEVVFP ALWRREPVDP AGGSGGSADP
61GWVRGVGGGG SARAQAAGSS REVRSVAPVP LEEPVEGRSE SRLRPPPPSE GEEDEELESQ
121ELPRGSSGAA ALSPGAPASW QPPPPPQPPP SPPPAQHAEP DGDEVLLRIP AFSRDLYLLL
181RRDGRFLAPR FAVEQRPNPG PGPTGAASAP QPPAPPDAGC FYTGAVLRHP GSLASFSTCG
241GGLMGFIQLN EDFIFIEPLN DTMAITGHPH RVYRQKRSME EKVTEKSALH SHYCGIISDK
301GRPRSRKIAE SGRGKRYSYK LPQEYNIETV VVADPAMVSY HGADAARRFI LTILNMVFNL
361FQHKSLSVQV NLRVIKLILL HETPPELYIG HHGEKMLESF CKWQHEEFGK KNDIHLEMST
421NWGEDMTSVD AAILITRKDF CVHKDEPCDT VGIAYLSGMC SEKRKCIIAE DNGLNLAFTI
481AHEMGHNMGI NHDNDHPSCA DGLHIMSGEW IKGQNLGDVS WSRCSKEDLE RFLRSKASNC
541LLQTNPQSVN SVMVPSKLPG MTYTADEQCQ ILFGPLASFC QEMQHVICTG LWCKVEGEKE
601CRTKLDPPMD GTDCDLGKWC KAGECTSRTS APEHLAGEWS LWSPCSRTCS AGISSRERKC
661PGLDSEARDC NGPRKQYRIC ENPPCPAGLP GFRDWQCQAY SVRTSSPKHI LQWQAVLDEE
721KPCALFCSPV GKEQPILLSE KVMDGTSCGY QGLDICANGR CQKVGCDGLL GSLAREDHCG
781VCNGNGKSCK IIKGDFNHTR GAGYVEVLVI PAGARRIKVV EEKPAHSYLA LRDAGKQSIN
841SDWKIEHSGA FNLAGTTVHY VRRGLWEKIS AKGPTTAPLH LLVLLFQDQN YGLHYEYTIP
901SDPLPENQSS KAPEPLFMWT HTSWEDCDAT CGGGERKTTV SCTKIMSKNI SIVDNEKCKY
961LTKPEPQIRK CNEQPCQTRW MMTEWTPCSR TCGKGMQSRQ VACTQQLSNG TLIRARERDC
1021IGPKPASAQR CEGQDCMTVW EAGVWSECSV KCGKGIRHRT VRCTNPRKKC VLSTRPREAE
1081DCEDYSKCYV WRMGDWSKCS ITCGKGMQSR VIQCMHKITG RHGNECFSSE KPAAYRPCHL
1141QPCNEKINVN TITSPRLAAL TFKCLGDQWP VYCRVIREKN LCQDMRWYQR CCETCRDFYA
1201QKLQQKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1207 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.