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ADAMTS16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTS16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAMTS16 antibodies →
$725.00each
Catalog Number: LS-G107116-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTS16 (NM_139056), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-TS16 | ADAM-TS 16 | ADAMTS16s | ADAMTS-16 | ADAMTS16 | KIAA2029
Origin Species
Human
NCBI GenBank
NM_139056
SwissProt
Q8TE57

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPRARGWRG LAALWMLLAQ VAEQAPACAM GPAAAAPGSP SVPRPPPPAE RPGWMEKGEY
61DLVSAYEVDH RGDYVSHEIM HHQRRRRAVP VSEVESLHLR LKGSRHDFHM DLRTSSSLVA
121PGFIVQTLGK TGTKSVQTLP PEDFCFYQGS LRSHRNSSVA LSTCQGLSGM IRTEEADYFL
181RPLPSHLSWK LGRAAQGSSP SHVLYKRSTE PHAPGASEVL VTSRTWELAH QPLHSSDLRL
241GLPQKQHFCG RRKKYMPQPP KEDLFILPDE YKSCLRHKRS LLRSHRNEEL NVETLVVVDK
301KMMQNHGHEN ITTYVLTILN MVSALFKDGT IGGNINIAIV GLILLEDEQP GLVISHHADH
361TLSSFCQWQS GLMGKDGTRH DHAILLTGLD ICSWKNEPCD TLGFAPISGM CSKYRSCTIN
421EDTGLGLAFT IAHESGHNFG MIHDGEGNMC KKSEGNIMSP TLAGRNGVFS WSPCSRQYLH
481KFLSTAQAIC LADQPKPVKE YKYPEKLPGE LYDANTQCKW QFGEKAKLCM LDFKKDICKA
541LWCHRIGRKC ETKFMPAAEG TICGHDMWCR GGQCVKYGDE GPKPTHGHWS DWSSWSPCSR
601TCGGGVSHRS RLCTNPKPSH GGKFCEGSTR TLKLCNSQKC PRDSVDFRAA QCAEHNSRRF
661RGRHYKWKPY TQVEDQDLCK LYCIAEGFDF FFSLSNKVKD GTPCSEDSRN VCIDGICERV
721GCDNVLGSDA VEDVCGVCNG NNSACTIHRG LYTKHHHTNQ YYHMVTIPSG ARSIRIYEMN
781VSTSYISVRN ALRRYYLNGH WTVDWPGRYK FSGTTFDYRR SYNEPENLIA TGPTNETLIV
841ELLFQGRNPG VAWEYSMPRL GTEKQPPAQP SYTWAIVRSE CSVSCGGGQM TVREGCYRDL
901KFQVNMSFCN PKTRPVTGLV PCKVSACPPS WSVGNWSACS RTCGGGAQSR PVQCTRRVHY
961DSEPVPASLC PQPAPSSRQA CNSQSCPPAW SAGPWAECSH TCGKGWRKRA VACKSTNPSA
1021RAQLLPDAVC TSEPKPRMHE ACLLQRCHKP KKLQWLVSAW SQCSVTCERG TQKRFLKCAE
1081KYVSGKYREL ASKKCSHLPK PSLELERACA PLPCPRHPPF AAAGPSRGSW FASPWSQCTA
1141SCGGGVQTRS VQCLAGGRPA SGCLLHQKPS ASLACNTHFC PIAEKKDAFC KDYFHWCYLV
1201PQHGMCSHKF YGKQCCKTCS KSNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1224 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.