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ADAMTS15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTS15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAMTS15 antibodies →
$725.00each
Catalog Number: LS-G107115-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTS15 (NM_139055), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-TS15 | ADAM-TS 15 | ADAMTS-15 | ADAMTS15
Origin Species
Human
NCBI GenBank
NM_139055
SwissProt
Q8TE58

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLLGILTLA FAGRTAGGSE PEREVVVPIR LDPDINGRRY YWRGPEDSGD QGLIFQITAF
61QEDFYLHLTP DAQFLAPAFS TEHLGVPLQG LTGGSSDLRR CFYSGDVNAE PDSFAAVSLC
121GGLRGAFGYR GAEYVISPLP NASAPAAQRN SQGAHLLQRR GVPGGPSGDP TSRCGVASGW
181NPAILRALDP YKPRRAGFGE SRSRRRSGRA KRFVSIPRYV ETLVVADESM VKFHGADLEH
241YLLTLLATAA RLYRHPSILN PINIVVVKVL LLRDRDSGPK VTGNAALTLR NFCAWQKKLN
301KVSDKHPEYW DTAILFTRQD LCGATTCDTL GMADVGTMCD PKRSCSVIED DGLPSAFTTA
361HELGHVFNMP HDNVKVCEEV FGKLRANHMM SPTLIQIDRA NPWSACSAAI ITDFLDSGHG
421DCLLDQPSKP ISLPEDLPGA SYTLSQQCEL AFGVGSKPCP YMQYCTKLWC TGKAKGQMVC
481QTRHFPWADG TSCGEGKLCL KGACVERHNL NKHRVDGSWA KWDPYGPCSR TCGGGVQLAR
541RQCTNPTPAN GGKYCEGVRV KYRSCNLEPC PSSASGKSFR EEQCEAFNGY NHSTNRLTLA
601VAWVPKYSGV SPRDKCKLIC RANGTGYFYV LAPKVVDGTL CSPDSTSVCV QGKCIKAGCD
661GNLGSKKRFD KCGVCGGDNK SCKKVTGLFT KPMHGYNFVV AIPAGASSID IRQRGYKGLI
721GDDNYLALKN SQGKYLLNGH FVVSAVERDL VVKGSLLRYS GTGTAVESLQ ASRPILEPLT
781VEVLSVGKMT PPRVRYSFYL PKEPREDKSS HPKDPRGPSV LHNSVLSLSN QVEQPDDRPP
841ARWVAGSWGP CSASCGSGLQ KRAVDCRGSA GQRTVPACDA AHRPVETQAC GEPCPTWELS
901AWSPCSKSCG RGFQRRSLKC VGHGGRLLAR DQCNLHRKPQ ELDFCVLRPC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

950 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.