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ADAMTS14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAMTS14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107097-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAMTS14 (NM_139155), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-TS14 | ADAMTS-14 | ADAMTS14 | ADAM-TS 14
Origin Species
Human
NCBI GenBank
NM_139155
SwissProt
Q8WXS8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPLRALLSY LLPLHCALCA AAGSRTPELH LSGKLSDYGV TVPCSTDFRG RFLSHVVSGP
61AAASAGSMVV DTPPTLPRHS SHLRVARSPL HPGGTLWPGR VGRHSLYFNV TVFGKELHLR
121LRPNRRLVVP GSSVEWQEDF RELFRQPLRQ ECVYTGGVTG MPGAAVAISN CDGLAGLIRT
181DSTDFFIEPL ERGQQEKEAS GRTHVVYRRE AVQQEWAEPD GDLHNEAFGL GDLPNLLGLV
241GDQLGDTERK RRHAKPGSYS IEVLLVVDDS VVRFHGKEHV QNYVLTLMNI VDEIYHDESL
301GVHINIALVR LIMVGYRQSL SLIERGNPSR SLEQVCRWAH SQQRQDPSHA EHHDHVVFLT
361RQDFGPSGMQ GYAPVTGMCH PLRSCALNHE DGFSSAFVIA HETGHVLGME HDGQGNGCAD
421ETSLGSVMAP LVQAAFHRFH WSRCSKLELS RYLPSYDCLL DDPFDPAWPQ PPELPGINYS
481MDEQCRFDFG SGYQTCLAFR TFEPCKQLWC SHPDNPYFCK TKKGPPLDGT ECAPGKWCFK
541GHCIWKSPEQ TYGQDGGWSS WTKFGSCSRS CGGGVRSRSR SCNNPSPAYG GRLCLGPMFE
601YQVCNSEECP GTYEDFRAQQ CAKRNSYYVH QNAKHSWVPY EPDDDAQKCE LICQSADTGD
661VVFMNQVVHD GTRCSYRDPY SVCARGECVP VGCDKEVGSM KADDKCGVCG GDNSHCRTVK
721GTLGKASKQA GALKLVQIPA GARHIQIEAL EKSPHRIVVK NQVTGSFILN PKGKEATSRT
781FTAMGLEWED AVEDAKESLK TSGPLPEAIA ILALPPTEGG PRSSLAYKYV IHEDLLPLIG
841SNNVLLEEMD TYEWALKSWA PCSKACGGGI QFTKYGCRRR RDHHMVQRHL CDHKKRPKPI
901RRRCNQHPCS QPVWVTEEWG ACSRSCGKLG VQTRGIQCLL PLSNGTHKVM PAKACAGDRP
961EARRPCLRVP CPAQWRLGAW SQCSATCGEG IQQRQVVCRT NANSLGHCEG DRPDTVQVCS
1021LPACGGNHQN STVRADVWEL GTPEGQWVPQ SEPLHPINKI SSTEPCTGDR SVFCQMEVLD
1081RYCSIPGYHR LCCVSCIKKA SGPNPGPDPG PTSLPPFSTP GSPLPGPQDP ADAAEPPGKP
1141TGSEDHQHGR ATQLPGALDT SSPGTQHPFA PETPIPGASW SISPTTPGGL PWGWTQTPTP
1201VPEDKGQPGE DLRHPGTSLP AASPVT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1226 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.