LSBio
ADAM32 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAM32 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAM32 antibodies →
$504.00each
Catalog Number: LS-G106855-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAM32 (NM_145004), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM 32 | ADAM32
Origin Species
Human
SwissProt
Q8TC27

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFRLWLLLAG LCGLLASRPG FQNSLLQIVI PEKIQTNTND SSEIEYEQIS YIIPIDEKLY
61TVHLKQRYFL ADNFMIYLYN QGSMNTYSSD IQTQCYYQGN IEGYPDSMVT LSTCSGLRGI
121LQFENVSYGI EPLESAVEFQ HVLYKLKNED NDIAIFIDRS LKEQPMDDNI FISEKSEPAV
181PDLFPLYLEM HIVVDKTLYD YWGSDSMIVT NKVIEIVGLA NSMFTQFKVT IVLSSLELWS
241DENKISTVGE ADELLQKFLE WKQSYLNLRP HDIAYLLIYM DYPRYLGAVF PGTMCITRYS
301AGVALYPKEI TLEAFAVIVT QMLALSLGIS YDDPKKCQCS ESTCIMNPEV VQSNGVKTFS
361SCSLRSFQNF ISNVGVKCLQ NKPQMQKKSP KPVCGNGRLE GNEICDCGTE AQCGPASCCD
421FRTCVLKDGA KCYKGLCCKD CQILQSGVEC RPKAHPECDI AENCNGSSPE CGPDITLING
481LSCKNNKFIC YDGDCHDLDA RCESVFGKGS RNAPFACYEE IQSQSDRFGN CGRDRNNKYV
541FCGWRNLICG RLVCTYPTRK PFHQENGDVI YAFVRDSVCI TVDYKLPRTV PDPLAVKNGS
601QCDIGRVCVN RECVESRIIK ASAHVCSQQC SGHGVCDSRN KCHCSPGYKP PNCQIRSKGF
661SIFPEEDMGS IMERASGKTE NTWLLGFLIA LPILIVTTAI VLARKQLKKW FAKEEEFPSS
721ESKSEGSTQT YASQSSSEGS TQTYASQTRS ESSSQADTSK SKSEDSAEAY TSRSKSQDST
781QTQSSSN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

787 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.