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ADAM30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAM30 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAM30 antibodies →
$504.00each
Catalog Number: LS-G101761-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAM30 (NM_021794), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM30 | ADAM 30 | Svph4
Origin Species
Human
NCBI GenBank
AF171932
SwissProt
Q9UKF2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRSVQIFLSQ CRLLLLLVPT MLLKSLGEDV IFHPEGEFDS YEVTIPEKLS FRGEVQGVVS
61PVSYLLQLKG KKHVLHLWPK RLLLPRHLRV FSFTEHGELL EDHPYIPKDC NYMGSVKESL
121DSKATISTCM GGLRGVFNID AKHYQIEPLK ASPSFEHVVY LLKKEQFGNQ VCGLSDDEIE
181WQMAPYENKA RLRDFPGSYK HPKYLELILL FDQSRYRFVN NNLSQVIHDA ILLTGIMDTY
241FQDVRMRIHL KALEVWTDFN KIRVGYPELA EVLGRFVIYK KSVLNARLSS DWAHLYLQRK
301YNDALAWSFG KVCSLEYAGS VSTLLDTNIL APATWSAHEL GHAVGMSHDE QYCQCRGRLN
361CIMGSGRTGF SNCSYISFFK HISSGATCLN NIPGLGYVLK RCGNKIVEDN EECDCGSTEE
421CQKDRCCQSN CKLQPGANCS IGLCCHDCRF RPSGYVCRQE GNECDLAEYC DGNSSSCPND
481VYKQDGTPCK YEGRCFRKGC RSRYMQCQSI FGPDAMEAPS ECYDAVNLIG DQFGNCEITG
541IRNFKKCESA NSICGRLQCI NVETIPDLPE HTTIISTHLQ AENLMCWGTG YHLSMKPMGI
601PDLGMINDGT SCGEGRVCFK KNCVNSSVLQ FDCLPEKCNT RGVCNNRKNC HCMYGWAPPF
661CEEVGYGGSI DSGPPGLLRG AIPSSIWVVS IIMFRLILLI LSVVFVFFRQ VIGNHLKPKQ
721EKMPLSKAKT EQEESKTKTV QEESKTKTGQ EESEAKTGQE ESKAKTGQEE SKANIESERP
781KAKSVKKQKK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

790 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.