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ADAM29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAM29 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAM29 antibodies →
$725.00each
Catalog Number: LS-G125096-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADAM29 (NM_001130704), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM 29 | Cancer/testis antigen 73 | CT73 | ADAM29 | Svph1
Origin Species
Human
NCBI GenBank
NM_014269
SwissProt
Q9UKF5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMLLLLHCL GVFLSCSGHI QDEHPQYHSP PDVVIPVRIT GTTRGMTPPG WLSYILPFGG
61QKHIIHIKVK KLLFSKHLPV FTYTDQGAIL EDQPFVQNNC YYHGYVEGDP ESLVSLSTCF
121GGFQGILQIN DFAYEIKPLA FSTTFEHLVY KMDSEEKQFS TMRSGFMQNE ITCRMEFEEI
181DNSTQKQSSY VGWWIHFRIV EIVVVIDNYL YIRYERNDSK LLEDLYVIVN IVDSILDVIG
241VKVLLFGLEI WTNKNLIVVD DVRKSVHLYC KWKSENITPR MQHDTSHLFT TLGLRGLSGI
301GAFRGMCTPH RSCAIVTFMN KTLGTFSIAV AHHLGHNLGM NHDEDTCRCS QPRCIMHEGN
361PPITKFSNCS YGDFWEYTVE RTKCLLETVH TKDIFNVKRC GNGVVEEGEE CDCGPLKHCA
421KDPCCLSNCT LTDGSTCAFG LCCKDCKFLP SGKVCRKEVN ECDLPEWCNG TSHKCPDDFY
481VEDGIPCKER GYCYEKSCHD RNEQCRRIFG AGANTASETC YKELNTLGDR VGHCGIKNAT
541YIKCNISDVQ CGRIQCENVT EIPNMSDHTT VHWARFNDIM CWSTDYHLGM KGPDIGEVKD
601GTECGIDHIC IHRHCVHITI LNSNCSPAFC NKRGICNNKH HCHCNYLWDP PNCLIKGYGG
661SVDSGPPPKR KKKKKFCYLC ILLLIVLFIL LCCLYRLCKK SKPIKKQQDV QTPSAKEEEK
721IQRRPHELPP QSQPWVMPSQ SQPPVTPSQS HPQVMPSQSQ PPVTPSQSQP RVMPSQSQPP
781VMPSQSHPQL TPSQSQPPVT PSQRQPQLMP SQSQPPVTPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

820 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.