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ADAD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADAD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADAD1 antibodies →
$504.00each
Catalog Number: LS-G128228-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TENR(ADAD1) (NM_001159295), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAD1 | Tenr
Origin Species
Human
SwissProt
Q96M93

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLKKNLPVQP ATKTITTPTG WSSESYGLSK MASKVTQVTG NFPEPLLSKN LSSISNPVLP
61PKKIPKEFIM KYKRGEINPV SALHQFAQMQ RVQLDLKETV TTGNVMGPYF AFCAVVDGIQ
121YKTGLGQNKK ESRSNAAKLA LDELLQLDEP EPRILETSGP PPFPAEPVVL SELAYVSKVH
181YEGRHIQYAK ISQIVKERFN QLISNRSEYL KYSSSLAAFI IERAGQHEVV AIGTGEYNYS
241QDIKPDGRVL HDTHAVVTAR RSLLRYFYRQ LLLFYSKNPA MMEKSIFCTE PTSNLLTLKQ
301NINICLYMNQ LPKGSAQIKS QLRLNPHSIS AFEANEELCL HVAVEGKIYL TVYCPKDGVN
361RISSMSSSDK LTRWEVLGVQ GALLSHFIQP VYISSILIGD GNCSDTRGLE IAIKQRVDDA
421LTSKLPMFYL VNRPHISLVP SAYPLQMNLE YKFLSLNWAQ GDVSLEIVDG LSGKITESSP
481FKSGMSMASR LCKAAMLSRF NLLAKEAKKE LLEAGTYHAA KCMSASYQEA KCKLKSYLQQ
541HGYGSWIVKS PCIEQFNM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.