Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACTR2 / ARP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122208-100
Catalog Number: LS-G122208
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Arp2(ACTR2) (NM_001005386), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Actin-like protein 2 | Actin-related protein 2 | ACTR2 | ARP2 | Actin-related protein arp2
- Origin Species
- Human
- NCBI GenBank
- NM_005722
- SwissProt
- P61160
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI EIKNNKKMDL61MVGDEASELR SMLEVNYPME NGIVRNWDDM KHLWDYTFGP EKLNIDTRNC KILLTEPPMN121PTKNREKIVE VMFETYQFSG VYVAIQAVLT LYAQGLLTGV VVDSGDGVTH ICPVYEGFSL181PHLTRRLDIA GRDITRYLIK LLLLRGYAFN HSADFETVRM IKEKLCYVGY NIEQEQKLAL241ETTVLVESYT LPDGRIIKVG GERFEAPEAL FQPHLINVEG VGVAELLFNT IQAADIDTRS301EFYKHIVLSG GSTMYPGLPS RLERELKQLY LERVLKGDVE KLSKFKIRIE DPPRRKHMVF361LGGAVLADIM KDKDNFWMTR QEYQEKGVRV LEKLGVTVRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.