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ACTR10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACTR10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101573-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACTR10 (NM_018477), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Actin-related protein 10 | ACTR11 | Arp11 | Actin-related protein 11 | ACTR10 | HARP11
Origin Species
Human
NCBI GenBank
NM_018477
SwissProt
Q9NZ32

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MPLYEGLGSG GEKTAVVIDL GEAFTKCGFA GETGPRCIIP SVIKRAGMPK PVRVVQYNIN
61TEELYSYLKE FIHILYFRHL LVNPRDRRVV IIESVLCPSH FRETLTRVLF KYFEVPSVLL
121APSHLMALLT LGINSAMVLD CGYRESLVLP IYEGIPVLNC WGALPLGGKA LHKELETQLL
181EQCTVDTSVA KEQSLPSVMG SVPEGVLEDI KARTCFVSDL KRGLKIQAAK FNIDGNNERP
241SPPPNVDYPL DGEKILHILG SIRDSVVEIL FEQDNEEQSV ATLILDSLIQ CPIDTRKQLA
301ENLVVIGGTS MLPGFLHRLL AEIRYLVEKP KYKKALGTKT FRIHTPPAKA NCVAWLGGAI
361FGALQDILGS RSVSKEYYNQ TGRIPDWCSL NNPPLEMMFD VGKTQPPLMK RAFSTEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

417 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.