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ACSS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACSS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109882-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACSS3 (NM_024560), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACSS3 | AMP-binding enzyme | 33217
Origin Species
Human
NCBI GenBank
NM_024560
SwissProt
Q9H6R3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPSWLQCRK VTSAGGLGGP LPGSSPARGA GAALRALVVP GPRGGLGGRG CRALSSGSGS
61EYKTHFAASV TDPERFWGKA AEQISWYKPW TKTLENKHSP STRWFVEGML NICYNAVDRH
121IENGKGDKIA IIYDSPVTNT KATFTYKEVL EQVSKLAGVL VKHGIKKGDT VVIYMPMIPQ
181AMYTMLACAR IGAIHSLIFG GFASKELSSR IDHVKPKVVV TASFGIEPGR RVEYVPLVEE
241ALKIGQHKPD KILIYNRPNM EAVPLAPGRD LDWDEEMAKA QSHDCVPVLS EHPLYILYTS
301GTTGLPKGVI RPTGGYAVML HWSMSSIYGL QPGEVWWAAS DLGWVVGHSY ICYGPLLHGN
361TTVLYEGKPV GTPDAGAYFR VLAEHGVAAL FTAPTAIRAI RQQDPGAALG KQYSLTRFKT
421LFVAGERCDV ETLEWSKNVF RVPVLDHWWQ TETGSPITAS CVGLGNSKTP PPGQAGKSVP
481GYNVMILDDN MQKLKARCLG NIVVKLPLPP GAFSGLWKNQ EAFKHLYFEK FPGYYDTMDA
541GYMDEEGYLY VMSRVDDVIN VAGHRISAGA IEESILSHGT VADCAVVGKE DPLKGHVPLA
601LCVLRKDINA TEEQVLEEIV KHVRQNIGPV AAFRNAVFVK QLPKTRSGKI PRSALSAIVN
661GKPYKITSTI EDPSIFGHVE EMLKQA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.