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ACSM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACSM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ACSM4 antibodies →
$725.00each
Catalog Number: LS-G119329-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACSM4 (NM_001080454), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACSM4
Origin Species
Human
SwissProt
P0C7M7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKIFFRYQTF RFIWLTKPPG RRLHKDHQLW TPLTLADFEA INRCNRPLPK NFNFAADVLD
61QWSQKEKTGE RPANPALWWV NGKGDEVKWS FRELGSLSRK AANVLTKPCG LQRGDRLAVI
121LPRIPEWWLV NVACIRTGII FMPGTIQLTA KDILYRLRAS KAKCIVASEE VAPAVESIVL
181ECPDLKTKLL VSPQSWNGWL SFQELFQFAS EEHSCVETGS QEPMTIYFTS GTTGFPKMAQ
241HSQSSLGIGF TLCGRYWLDL KSSDIIWNMS DTGWVKAAIG SVFSSWLCGA CVFVHRMAQF
301DTDTFLDTLT TYPITTLCSP PTVYRMLVQK DLKRYKFKSL RHCLTGGEPL NPEVLEQWRV
361QTGLELYEGY GQTEVGMICA NQKGQEIKPG SMGKGMLPYD VQIIDENGNV LPPGKEGEIA
421LRLKPTRPFC FFSKYVDNPQ KTAATIRGDF YVTGDRGVMD SDGYFWFVGR ADDVIISSGY
481RIGPFEVESA LIEHPAVVES AVVSSPDQIR GEVVKAFVVL AAPFKSYNPE KLTLELQDHV
541KKSTAPYKYP RKVEFVQELP KTITGKIKRN VLRDQEWRGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

580 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.