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ACSL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACSL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ACSL6 antibodies →
$725.00each
Catalog Number: LS-G121435-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACSL6 (NM_001009185), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACS2 | ACSL6 | FACL6 | LACS 6 | LACS2 | KIAA0837 | LACS5
Origin Species
Human
NCBI GenBank
AB020644
SwissProt
Q9UKU0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTFFLVSGG SLWLFVEFVL SLLEKMQTQE ILRILRLPEL GDLGQFFRSL SATTLVSMGA
61LAAILAYWFT HRPKALQPPC NLLMQSEEVE DSGGARRSVI GSGPQLLTHY YDDARTMYQV
121FRRGLSISGN GPCLGFRKPK QPYQWLSYQE VADRAEFLGS GLLQHNCKAC TDQFIGVFAQ
181NRPEWIIVEL ACYTYSMVVV PLYDTLGPGA IRYIINTADI STVIVDKPQK AVLLLEHVER
241KETPGLKLII LMDPFEEALK ERGQKCGVVI KSMQAVEDCG QENHQAPVPP QPDDLSIVCF
301TSGTTGNPKG AMLTHGNVVA DFSGFLKVTE KVIFPRQDDV LISFLPLAHM FERVIQSVVY
361CHGGRVGFFQ GDIRLLSDDM KALCPTIFPV VPRLLNRMYD KIFSQANTPL KRWLLEFAAK
421RKQAEVRSGI IRNDSIWDEL FFNKIQASLG GCVRMIVTGA APASPTVLGF LRAALGCQVY
481EGYGQTECTA GCTFTTPGDW TSGHVGAPLP CNHIKLVDVE ELNYWACKGE GEICVRGPNV
541FKGYLKDPDR TKEALDSDGW LHTGDIGKWL PAGTLKIIDR KKHIFKLAQG EYVAPEKIEN
601IYIRSQPVAQ IYVHGDSLKA FLVGIVVPDP EVMPSWAQKR GIEGTYADLC TNKDLKKAIL
661EDMVRLGKES GLHSFEQVKA IHIHSDMFSV QNGLLTPTLK AKRPELREYF KKQIEELYSI
721SM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

722 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.