Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACSBG1 / hsBG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113422-100
Catalog Number: LS-G113422
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACSBG1 (NM_015162), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACSBG1 | BG1 | Bubblegum | BG | BGM | KIAA0631 | Lipidosin | LPD | HsBG | HsBGM | GR-LACS
- Origin Species
- Human
- NCBI GenBank
- NM_015162
- SwissProt
- Q96GR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRNSGAGYG CPHGDPSMLD SRETPQESRQ DMIVRTTQEK LKTSSLTDRQ PLSKESLNHA61LELSVPEKVN NAQWDAPEEA LWTTRADGRV RLRIDPSCPQ LPYTVHRMFY EALDKYGDLI121ALGFKRQDKW EHISYSQYYL LARRAAKGFL KLGLKQAHSV AILGFNSPEW FFSAVGTVFA181GGIVTGIYTT SSPEACQYIA YDCCANVIMV DTQKQLEKIL KIWKQLPHLK AVVIYKEPPP241NKMANVYTME EFMELGNEVP EEALDAIIDT QQPNQCCVLV YTSGTTGNPK GVMLSQDNIT301WTARYGSQAG DIRPAEVQQE VVVSYLPLSH IAAQIYDLWT GIQWGAQVCF AEPDALKGSL361VNTLREVEPT SHMGVPRVWE KIMERIQEVA AQSGFIRRKM LLWAMSVTLE QNLTCPGSDL421KPFTTRLADY LVLAKVRQAL GFAKCQKNFY GAAPMMAETQ HFFLGLNIRL YAGYGLSETS481GPHFMSSPYN YRLYSSGKLV PGCRVKLVNQ DAEGIGEICL WGRTIFMGYL NMEDKTCEAI541DEEGWLHTGD AGRLDADGFL YITGRLKELI ITAGGENVPP VPIEEAVKME LPIISNAMLI601GDQRKFLSML LTLKCTLDPD TSDQTDNLTE QAVEFCQRVG SRATTVSEII EKKDEAVYQA661IEEGIRRVNM NAAARPYHIQ KWAILERDFS ISGGELGPTM KLKRLTVLEK YKGIIDSFYQ721EQKMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
724 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.