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ACOT9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACOT9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120486-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Acyl CoA Thioesterase 9(ACOT9) (NM_001037171), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACOT9 | ACATE2 | Acyl-CoA thioester hydrolase 9 | Acyl-CoA thioesterase 9 | MTACT48 | CGI-16 | MT-ACT48
Origin Species
Human
NCBI GenBank
AF132950
SwissProt
Q9Y305

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.85 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRAALRLCA LGKGQLTPGR GLTQGPQNPK KQGIFHIHEA CSSIHVNHVR DKLREIVGAS
61TNWRDHVKAM EERKLLHSFL AKSQDGLPPR RMKDSYIEVL LPLGSEPELR EKYLTVQNTV
121RFGRILEDLD SLGVLICYMH NKIHSAKMSP LSIVTALVDK IDMCKKSLSP EQDIKFSGHV
181SWVGKTSMEV KMQMFQLHGD EFCPVLDATF VMVARDSENK GPAFVNPLIP ESPEEEELFR
241QGELNKGRRI AFSSTSLLKM APSAEERTTI HEMFLSTLDP KTISFRSRVL PSNAVWMENS
301KLKSLEICHP QERNIFNRIF GGFLMRKAYE LAWATACSFG GSRPFVVAVD DIMFQKPVEV
361GSLLFLSSQV CFTQNNYIQV RVHSEVASLQ EKQHTTTNVF HFTFMSEKEV PLVFPKTYGE
421SMLYLDGQRH FNSMSGPATL RKDYLVEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

448 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.