Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACKR2 / CCR10 / D6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99402-100
Catalog Number: LS-G99402
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Chemokine Receptor D6(ACKR2) (NM_001296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-C chemokine receptor D6 | CCBP2 | Chemokine (C-C) receptor 9 | Chemokine binding protein 2 | C-C chemokine receptor 10 | Chemokine-binding protein 2 | CMKBR9 | Chemokine receptor CCR-9 | G protein coupled receptor d6 | HD6 | Cc-chemokine receptor type 10 | Chemokine receptor CCR-10 | Chemokine receptor D6 | Chemokine-binding protein D6 | D6
- NCBI GenBank
- NM_001296
- SwissProt
- O00590
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAATASPQPL ATEDADSENS SFYYYDYLDE VAFMLCRKDA VVSFGKVFLP VFYSLIFVLG61LSGNLLLLMV LLRYVPRRRM VEIYLLNLAI SNLLFLVTLP FWGISVAWHW VFGSFLCKMV121STLYTINFYS GIFFISCMSL DKYLEIVHAQ PYHRLRTRAK SLLLATIVWA VSLAVSIPDM181VFVQTHENPK GVWNCHADFG GHGTIWKLFL RFQQNLLGFL LPLLAMIFFY SRIGCVLVRL241RPAGQGRALK IAAALVVAFF VLWFPYNLTL FLHTLLDLQV FGNCEVSQHL DYALQVTESI301AFLHCCFSPI LYAFSSHRFR QYLKAFLAAV LGWHLAPGTA QASLSSCSES SILTAQEEMT361GMNDLGERQS ENYPNKEDVG NKSATRTRPL EQKLISEEDL AANDILDYKD DDDKV
415 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.