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ACCSL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACCSL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ACCSL antibodies →
$725.00each
Catalog Number: LS-G120757-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACCSL (NM_001031854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACCSL | ACC synthase-like protein 2
Origin Species
Human
SwissProt
Q4AC99

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSHRSDTLPV PSGQRRGRVP RDHSIYTQLL EITLHLQQAM TEHFVQLTSR QGLSLEERRH
61TEAICEHEAL LSRLICRMIN LLQSGAASGL ELQVPLPSED SRGDVRYGQR AQLSGQPDPV
121PQLSDCEAAF VNRDLSIRGI DISVFYQSSF QDYNAYQKDK YHKDKNTLGF INLGTSENKL
181CMDLMTERLQ ESDMNCIEDT LLQYPDWRGQ PFLREEVARF LTYYCRAPTR LDPENVVVLN
241GCCSVFCALA MVLCDPGEAF LVPAPFYGGF AFSSRLYAKV ELIPVHLESE VTVTNTHPFQ
301LTVDKLEEAL LEARLEGKKV RGLVLINPQN PLGDIYSPDS LMKYLEFAKR YNLHVIIDEI
361YMLSVFDESI TFHSILSMKS LPDSNRTHVI WGTSKDFGIS GFRFGALYTH NKEVASAVSA
421FGYLHSISGI TQHKLCQLLQ NTEWIDKVYL PTNCYRLREA HKYITAELKA LEIPFHNRSS
481GLYVWINLKK YLDPCTFEEE RLLYCRFLDN KLLLSRGKTY MCKEPGWFCL IFADELPRLK
541LAMRRFCDVL QEQKEALIVK QLEDAMRE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

568 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.