Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACCS / ACS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108715-100
Catalog Number: LS-G108715
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHACS(ACCS) (NM_032592), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACS | ACC synthase-like protein 1 | ACCS | PHACS
- Origin Species
- Human
- NCBI GenBank
- NM_032592
- SwissProt
- Q96QU6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFTLPQKDFR APTTCLGPTC MQDLGSSHGE DLEGECSRKL DQKLPELRGV GDPAMISSDT61SYLSSRGRMI KWFWDSAEEG YRTYHMDEYD EDKNPSGIIN LGTSENKLCF DLLSWRLSQR121DMQRVEPSLL QYADWRGHLF LREEVAKFLS FYCKSPVPLR PENVVVLNGG ASLFSALATV181LCEAGEAFLI PTPYYGAITQ HVCLYGNIRL AYVYLDSEVT GLDTRPFQLT VEKLEMALRE241AHSEGVKVKG LILISPQNPL GDVYSPEELQ EYLVFAKRHR LHVIVDEVYM LSVFEKSVGY301RSVLSLERLP DPQRTHVMWA TSKDFGMSGL RFGTLYTENQ DVATAVASLC RYHGLSGLVQ361YQMAQLLRDR DWINQVYLPE NHARLKAAHT YVSEELRALG IPFLSRGAGF FIWVDLRKYL421LKGTFEEEML LWRRFLDNKV LLSFGKAFEC KEPGWFRFVF SDQVHRLCLG MQRVQQVLAG481KSQVAEDPRP SQSQEPSDQR RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
501 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.