Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACCN5 / HINAC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112450-100
Catalog Number: LS-G112450
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACCN5(ASIC5) (NM_017419), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Acid-sensing ion channel 5 | BLINaC | ACCN5 | HINAC | Human intestine Na(+) channel | INAC | ASIC5
- Origin Species
- Human
- NCBI GenBank
- AJ252011
- SwissProt
- Q9NY37
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQTEKSKVY AENGLLEKIK LCPSKKPLPS PTERKKFDYD FAISTSFHGI HNIVQNRSKI61RRVLWLVVVL GSVSLVTWQI YIRLLNYFTW PTTASIEVQY VEKMEFPTVT FCNLNRFQTD121AVAKFGVIFF LWHIVSKVLH LQEITANSTG SREATDFAAS HQNFSIVEFI RNKGFYLNNS181TLLDCEFFGK PCSPKDFAHV FTEYGNCFTF NHGETLQAKR KVSVSGRGLS LLFNVNQEAF241TDNPALGFVD AGIIFVIHSP KKVPQFDGLG LLSPVGMHAR VTIRQVKTVH QEYPWGECNP301NIKLQNFSSY STSGCLKECK AQHIKKQCGC VPFLLPGYGI ECDLQKYFSC VSPVLDHIEF361KDLCTVGTHN SSCPVSCEEI EYPATISYSS FPSQKALKYL SKKLNQSRKY IRENLVKIEI421NYSDLNYKIT QQQKAVSVSE LLADLGGQLG LFCGASLITI IEIIEYLFTN FYWICIFFLL481KISEMTQWTP PPQNHLGNKN RIEECTRTRPLEQKLISEEDLAANDILDYKDDDDKV
505 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.