Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACCN4 / ASIC4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104143-100
Catalog Number: LS-G104143
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACCN4(ASIC4) (NM_182847), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACCN4 | Acid-sensing ion channel 4 | ASIC4 | BNAC4 | Brain sodium channel 4 | Spasic
- Origin Species
- Human
- NCBI GenBank
- NM_018674
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSGAAGAAR RGGAALAPSL TRSLAGTHAG ADSCAGADKG SHKETIEERD KRQQRQQRQR61QHQGCGAAGS GSDSPTSGPH PVPVLFPLAL SLEEQPLPPL PLGRAPGLLA REGQGREALA121SPSSRGQMPI EIVCKIKFAE EDAKPKEKEA GDEQSLLGAV APGAAPRDLA TFASTSTLHG181LGRACGPGPH GLRRTLWALA LLTSLAAFLY QAAGLARGYL TRPHLVAMDP AAPAPVAGFP241AVTLCNINRF RHSALSDADI FHLANLTGLP PKDRDGHRAA GLRYPEPDMV DILNRTGHQL301ADMLKSCNFS GHHCSASNFS VVYTRYGKCY TFNADPRSSL PSRAGGMGSG LEIMLDIQQE361EYLPIWRETN ETSFEAGIRV QIHSQEEPPY IHQLGFGVSP GFQTFVSCQE QRLTYLPQPW421GNCRAESELR EPELQGYSAY SVSACRLRCE KEAVLQRCHC RMVHMPDSLG GGPEGPCFCP481TPCNLTRYGK EISMVRIPNR GSARYLARKY NRNETYIREN FLVLDVFFEA LTSEAMEQRA541AYGLSALLGD LGGQMGLFIG ASILTLLEIL DYIYEVSWDR LKRVWRRPKT PLRTSTGGIS601TLGLQELKEQ SPCPSLGRAE GGGVSSLLPN HHHPHGPPGG LFEDFACTRTRPLEQKLISEEDLAANDILDYKDDDDKV
647 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.