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ACAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129133-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACAP3 (NM_030649), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACAP3 | CENTB5 | Cnt-b5 | Centaurin | beta 5 | Centaurin-beta-5 | KIAA1716
Origin Species
Human
NCBI GenBank
R73374
SwissProt
Q96P50

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTVEFEECVK DSPRFRATID EVETDVVEIE AKLDKLVKLC SGMVEAGKAY VSTSRLFVSG
61VRDLSQQCQG DTVISECLQR FADSLQEVVN YHMILFDQAQ RSVRQQLQSF VKEDVRKFKE
121TKKQFDKVRE DLELSLVRNA QAPRHRPHEV EEATGALTLT RKCFRHLALD YVLQINVLQA
181KKKFEILDSM LSFMHAQSSF FQQGYSLLHQ LDPYMKKLAA ELDQLVIDSA VEKREMERKH
241AAIQQRTLLQ DFSYDESKVE FDVDAPSGVV MEGYLFKRAS NAFKTWNRRW FSIQNSQLVY
301QKKLKDALTV VVDDLRLCSV KPCEDIERRF CFEVLSPTKS CMLQADSEKL RQAWVQAVQA
361SIASAYRESP DSCYSERLDR TASPSTSSID SATDTRERGV KGESVLQRVQ SVAGNSQCGD
421CGQPDPRWAS INLGVLLCIE CSGIHRSLGV HCSKVRSLTL DSWEPELLKL MCELGNSAVN
481QIYEAQCEGA GSRKPTASSS RQDKEAWIKD KYVEKKFLRK APMAPALEAP RRWRVQKCLR
541PHSSPRAPTA RRKVRLEPVL PCVAALSSVG TLDRKFRRDS LFCPDELDSL FSYFDAGAAG
601AGPRSLSSDS GLGGSSDGSS DVLAFGSGSV VDSVTEEEGA ESEESSGEAD GDTEAEAWGL
661ADVRELHPGL LAHRAARARD LPALAAALAH GAEVNWADAE DEGKTPLVQA VLGGSLIVCE
721FLLQNGADVN QRDSRGRAPL HHATLLGRTG QVCLFLKRGA DQHALDQEQR DPLAIAVQAA
781NADIVTLLRL ARMAEEMREA EAAPGPPGAL AGSPTELQFR RCIQEFISLH LEES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

834 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.