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ACAD10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ACAD10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ACAD10 antibodies →
$504.00each
Catalog Number: LS-G109500-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACAD10 (NM_025247), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACAD-10 | ACAD10
Origin Species
Human
NCBI GenBank
NM_025247
SwissProt
Q6JQN1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
118.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCVRSCFQSP RLQWVWRTAF LKHTQRRHQG SHRWTHLGGS TYRAVIFDMG GVLIPSPGRV
61AAEWEVQNRI PSGTILKALM EGGENGPWMR FMRAEITAEG FLREFGRLCS EMLKTSVPVD
121SFFSLLTSER VAKQFPVMTE AITQIRAKGL QTAVLSNNFY LPNQKSFLPL DRKQFDVIVE
181SCMEGICKPD PRIYKLCLEQ LGLQPSESIF LDDLGTNLKE AARLGIHTIK VNDPETAVKE
241LEALLGFTLR VGVPNTRPVK KTMEIPKDSL QKYLKDLLGI QTTGPLELLQ FDHGQSNPTY
301YIRLANRDLV LRKKPPGTLL PSAHAIEREF RIMKALANAG VPVPNVLDLC EDSSVIGTPF
361YVMEYCPGLI YKDPSLPGLE PSHRRAIYTA MNTVLCKIHS VDLQAVGLED YGKQGDYIPR
421QVRTWVKQYR ASETSTIPAM ERLIEWLPLH LPRQQRTTVV HGDFRLDNLV FHPEEPEVLA
481VLDWELSTLG DPLADVAYSC LAHYLPSSFP VLRGINDCDL TQLGIPAAEE YFRMYCLQMG
541LPPTENWNFY MAFSFFRVAA ILQGVYKRSL TGQASSTYAE QTGKLTEFVS NLAWDFAVKE
601GFRVFKEMPF TNPLTRSYHT WARPQSQWCP TGSRSYSSVP EASPAHTSRG GLVISPESLS
661PPVRELYHRL KHFMEQRVYP AEPELQSHQA SAARWSPSPL IEDLKEKAKA EGLWNLFLPL
721EADPEKKYGA GLTNVEYAHL CELMGTSLYA PEVCNCSAPD TGNMELLVRY GTEAQKARWL
781IPLLEGKARS CFAMTEPQVA SSDATNIEAS IREEDSFYVI NGHKWWITGI LDPRCQLCVF
841MGKTDPHAPR HRQQSVLLVP MDTPGIKIIR PLTVYGLEDA PGGHGEVRFE HVRVPKENMV
901LGPGRGFEIA QGRLGPGRIH HCMRLIGFSE RALALMKARV KSRLAFGKPL VEQGTVLADI
961AQSRVEIEQA RLLVLRAAHL MDLAGNKAAA LDIAMIKMVA PSMASRVIDR AIQAFGAAGL
1021SSDYPLAQFF TWARALRFAD GPDEVHRATV AKLELKHRI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1059 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.