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ABTB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABTB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABTB2 antibodies →
$504.00each
Catalog Number: LS-G106576-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABTB2 (NM_145804), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABTB2 | BTBD22 | DKFZP586C1619
Origin Species
Human
NCBI GenBank
NM_145804

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAGDGLRRG KSARCGLTFS VGRFFRWMVD TRISVRIHEY AAISLTACME NLVEEIRARV
61MASHSPDGGG AGGGEVSAEA LEMVINNDAE LWGVLQPYEH LICGKNANGV LSLPAYFSPY
121NGGSLGHDER ADAYAQLELR TLEQSLLATC VGSISELSDL VSRAMHHMQG RHPLCPGASP
181ARQARQPPQP ITWSPDALHT LYYFLRCPQM ESMENPNLDP PRMTLNNERP FMLLPPLMEW
241MRVAITYAEH RRSLTVDSGD IRQAARLLLP GLDCEPRQLK PEHCFSSFRR LDARAATEKF
301NQDLGFRMLN CGRTDLINQA IEALGPDGVN TMDDQGMTPL MYACAAGDEA MVQMLIDAGA
361NLDIQVPSNS PRHPSIHPDS RHWTSLTFAV LHGHISVVQL LLDAGAHVEG SAVNGGEDSY
421AETPLQLASA AGNYELVSLL LSRGADPLLS MLEAHGMGSS LHEDMNCFSH SAAHGHRNVL
481RKLLTQPQQA KADVLSLEEI LAEGVEESDA SSQGSGSEGP VRLSRTRTKA LQEAMYYSAE
541HGYVDITMEL RALGVPWKLH IWIESLRTSF SQSRYSVVQS LLRDFSSIRE EEYNEELVTE
601GLQLMFDILK TSKNDSVIQQ LATIFTHCYG SSPIPSIPEI RKTLPARLDP HFLNNKEMSD
661VTFLVEGKLF YAHKVLLVTA SNRFKTLMTN KSEQDGDSSK TIEISDMKYH IFQMMMQYLY
721YGGTESMEIP TTDILELLSA ASLFQLDALQ RHCEILCSQT LSMESAVNTY KYAKIHNAPE
781LALFCEGFFL KHMKALLEQD AFRQLIYGRS SKVQGLDPLQ DLQNTLAERV HSVYITSRV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.