
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ABRI / ITM2B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110528-100
Catalog Number: LS-G110528
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITM2B (NM_021999), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ABri/ADan amyloid peptide | ABRI | BRI | BRI2 | E3-16 | Integral membrane protein 2B | ImBRI2 | FBD | Protein E25B | BRICD2B | BRICHOS domain containing 2B | Transmembrane protein BRI | E25B | Immature BRI2 | ITM2B
- Origin Species
- Human
- NCBI GenBank
- NM_021999
- SwissProt
- Q9Y287
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 30.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVKVTFNSAL AQKETKKDEP KSGEEALIIP PDAVAVDCKD PDDVVPVGQR RAWCWCMCFG61LAFMLAGVIL GGAYLYKYFA LQPDDVYYCG IKYIKDDVIL NEPSADAPAA LYQTIEENIK121IFEEEEVEFI SVPVPEFADS DPANIVHDFN KKLTAYLDLN LDKCYVIPLN TSIVMPPRNL181LELLINIKAG TYLPQSYLIH EHMVITDRIE NIDHLGFFIY RLCHDKETYK LQRRETIKGI241QKREASNCFA IRHFENKFAV ETLICSTRTR PLEQKLISEE DLAANDILDY KDDDDKV
297 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
