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ABLIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABLIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABLIM2 antibodies →
$725.00each
Catalog Number: LS-G108821-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABLIM2 (NM_032432), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Actin binding LIM protein 2 | AbLIM-2 | Actin-binding LIM protein 2 | ABLIM2 | KIAA1808
Origin Species
Human
SwissProt
Q6H8Q1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG
61GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD
121RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC
181KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR
241CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY
301AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGEG DQDDRSYKQC
361RTSSPSSTGS VSLGRYTPTS RSPQHYSRPA ARRSDGEDGS LDQDNRKQKS SWLMLKGDAD
421TRTNSPDLDT QSLSHSSGTD RDPLQRMAGD SFHSQYKIYP YDSLIVTNRI RVKLPKDVDR
481TRLERHLSPE EFQEVFGMSI EEFDRLALWK RNDLKKKALL F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

521 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.