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ABL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABL2 antibodies →
$725.00each
Catalog Number: LS-G118865-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABL2 (NM_001100108), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Abl-related gene ARG | ABLL | Abelson-related gene protein | ARG | ABL2 | Oncogene abl2 | Tyrosine-protein kinase ARG
Origin Species
Human
NCBI GenBank
NM_007314
SwissProt
P42684

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLGTVLLPP NSYGRDQDTS LCCLCTEASE SALPDLTDHF ASCVEDGFEG DKTGGSSPEA
61LHRPYGCDVE PQALNEAIRW SSKENLLGAT ESDPNLFVAL YDFVASGDNT LSITKGEKLR
121VLGYNQNGEW SEVRSKNGQG WVPSNYITPV NSLEKHSWYH GPVSRSAAEY LLSSLINGSF
181LVRESESSPG QLSISLRYEG RVYHYRINTT ADGKVYVTAE SRFSTLAELV HHHSTVADGL
241VTTLHYPAPK CNKPTVYGVS PIHDKWEMER TDITMKHKLG GGQYGEVYVG VWKKYSLTVA
301VKTLKEDTME VEEFLKEAAV MKEIKHPNLV QLLGVCTLEP PFYIVTEYMP YGNLLDYLRE
361CNREEVTAVV LLYMATQISS AMEYLEKKNF IHRDLAARNC LVGENHVVKV ADFGLSRLMT
421GDTYTAHAGA KFPIKWTAPE SLAYNTFSIK SDVWAFGVLL WEIATYGMSP YPGIDLSQVY
481DLLEKGYRME QPEGCPPKVY ELMRACWKWS PADRPSFAET HQAFETMFHD SSISEEVAEE
541LGRAASSSSV VPYLPRLPIL PSKTRTLKKQ VENKENIEGA QDATENSASS LAPGFIRGAQ
601ASSGSPALPR KQRDKSPSSL LEDAKETCFT RDRKGGFFSS FMKKRNAPTP PKRSSSFREM
661ENQPHKKYEL TGNFSSVASL QHADGFSFTP AQQEANLVPP KCYGGSFAQR NLCNDDGGGG
721GGSGTAGGGW SGITGFFTPR LIKKTLGLRA GKPTASDDTS KPFPRSNSTS SMSSGLPEQD
781RMAMTLPRNC QRSKLQLERT VSTSSQPEEN VDRANDMLPK KSEESAAPSR ERPKAKLLPR
841GATALPLRTP SGDLAITEKD PPGVGVAGVA AAPKGKEKNG GARLGMAGVP EDGEQPGWPS
901PAKAAPVLPT THNHKVPVLI SPTLKHTPAD VQLIGTDSQG NKFKLLSEHQ VTSSGDKDRP
961RRVKPKCAPP PPPVMRLLQH PSICSDPTEE PTALTAGQST SETQEGGKKA ALGAVPISGK
1021AGRPVMPPPQ VPLPTSSISP AKMANGTAGT KVALRKTKQA AEKISADKIS KEALLECADL
1081LSSALTEPVP NSQLVDTGHQ LLDYCSGYVD CIPQTRNKFA FREAVSKLEL SLQELQVSSA
1141AAGVPGTNPV LNNLLSCVQE ISDVVQR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1167 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.