Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ABL1 / c-ABL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114692-100
Catalog Number: LS-G114692
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human c Abl(ABL1) (NM_007313), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ABL1 | Bcr/c-abl oncogene protein | Bcr-Abl | Bcr/abl | C-ABL | ABL | JTK7 | p150 | Tyrosine-protein kinase ABL1 | Proto-oncogene c-Abl | V-abl
- Origin Species
- Human
- NCBI GenBank
- NM_005157
- SwissProt
- P00519
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 124.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQQPGKVLG DQRRPSLPAL HFIKGAGKKE SSRHGGPHCN VFVEHEALQR PVASDFEPQG61LSEAARWNSK ENLLAGPSEN DPNLFVALYD FVASGDNTLS ITKGEKLRVL GYNHNGEWCE121AQTKNGQGWV PSNYITPVNS LEKHSWYHGP VSRNAAEYLL SSGINGSFLV RESESSPGQR181SISLRYEGRV YHYRINTASD GKLYVSSESR FNTLAELVHH HSTVADGLIT TLHYPAPKRN241KPTVYGVSPN YDKWEMERTD ITMKHKLGGG QYGEVYEGVW KKYSLTVAVK TLKEDTMEVE301EFLKEAAVMK EIKHPNLVQL LGVCTREPPF YIITEFMTYG NLLDYLRECN RQEVNAVVLL361YMATQISSAM EYLEKKNFIH RDLAARNCLV GENHLVKVAD FGLSRLMTGD TYTAHAGAKF421PIKWTAPESL AYNKFSIKSD VWAFGVLLWE IATYGMSPYP GIDLSQVYEL LEKDYRMERP481EGCPEKVYEL MRACWQWNPS DRPSFAEIHQ AFETMFQESS ISDEVEKELG KQGVRGAVST541LLQAPELPTK TRTSRRAAEH RDTTDVPEMP HSKGQGESDP LDHEPAVSPL LPRKERGPPE601GGLNEDERLL PKDKKTNLFS ALIKKKKKTA PTPPKRSSSF REMDGQPERR GAGEEEGRDI661SNGALAFTPL DTADPAKSPK PSNGAGVPNG ALRESGGSGF RSPHLWKKSS TLTSSRLATG721EEEGGGSSSK RFLRSCSASC VPHGAKDTEW RSVTLPRDLQ STGRQFDSST FGGHKSEKPA781LPRKRAGENR SDQVTRGTVT PPPRLVKKNE EAADEVFKDI MESSPGSSPP NLTPKPLRRQ841VTVAPASGLP HKEEAGKGSA LGTPAAAEPV TPTSKAGSGA PGGTSKGPAE ESRVRRHKHS901SESPGRDKGK LSRLKPAPPP PPAASAGKAG GKPSQSPSQE AAGEAVLGAK TKATSLVDAV961NSDAAKPSQP GEGLKKPVLP ATPKPQSAKP SGTPISPAPV PSTLPSASSA LAGDQPSSTA1021FIPLISTRVS LRKTRQPPER IASGAITKGV VLDSTEALCL AISRNSEQMA SHSAVLEAGK1081NLYTFCVSYV DSIQQMRNKF AFREAINKLE NNLRELQICP ATAGSGPAAT QDFSKLLSSV1141KEISDIVQRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1149 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.