Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ABI1 / SSH3BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121362-100
Catalog Number: LS-G121362
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SSH3BP1(ABI1) (NM_001012750), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Abelson interactor 1 | ABI1 | Abl-interactor protein 1 long | ABI-1 | Abl-interactor 1 | Abl interactor 1 | Abl-binding protein 4 | ABLBP4 | NAP1BP | Nap1 binding protein | SSH3BP1 | Nap1-binding protein | E3B1 | Eps8-binding protein | Interactor protein AblBP4 | SSH3BP
- Origin Species
- Human
- NCBI GenBank
- NM_005470
- SwissProt
- Q8IZP0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET KAYTTQSLAS61VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH KEKVARREIG ILTTNKNTSR121THKIIAPANM ERPVRYIRKP IDYTVLDDVG HGVKWLKAKH GNNQPARTGT LSRTNPPTQK181PPSPPMSGRG TLGRNTPYKT LEPVKPPTVP NDYMTSPARL GSQHSPGRTA SLNQRPRTHS241GSSGGSGSRE NSGSSSIGIP IAVPTPSPPT IGPAAPGSAP GSQYGTMTRQ ISRHNSTTSS301TSSGGYRRTP SVTAQFSAQP HVNGGPLYSQ NSISIAPPPP PMPQLTPQIP LTGFVARVQE361NIADSPTPPP PPPPDDIPMF DDSPPPPPPP PVDYEDEEAA VVQYNDPYAD GDPAWAPKNY421IEKVVAIYDY TKDKDDELSF MEGAIIYVIK KNDDGWYEGV CNRVTGLFPG NYVESIMHYT481DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
481 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.