Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ABHD17B / FAM108B1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112927-100
Catalog Number: LS-G112927
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM108B1(ABHD17B) (NM_016014), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C9orf77 | CGI-67 | FAM108B1 | RP11-409O11.2
- Origin Species
- Human
- NCBI GenBank
- NM_016014
- SwissProt
- Q5VST6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNNLSFSELC CLFCCPPCPG KIASKLAFLP PDPTYTLMCD ESGSRWTLHL SERADWQYSS61REKDAIECFM TRTSKGNRIA CMFVRCSPNA KYTLLFSHGN AVDLGQMSSF YIGLGSRINC121NIFSYDYSGY GASSGKPTEK NLYADIEAAW LALRTRYGIR PENVIIYGQS IGTVPSVDLA181ARYESAAVIL HSPLTSGMRV AFPDTKKTYC FDAFPNIDKI SKITSPVLII HGTEDEVIDF241SHGLALFERC QRPVEPLWVE GAGHNDVELY GQYLERLKQF VSQELVQKHK EGKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
293 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.