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ABHD12B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABHD12B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABHD12B antibodies →
$504.00each
Catalog Number: LS-G104448-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABHD12B (NM_181533), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABHD12B | C14orf29 | BEM46L3 | C14_5314
Origin Species
Human
NCBI GenBank
NM_181533
SwissProt
Q7Z5M8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
28.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MLGIWHTVPS CRGEDAKGKD CCWYEAALRD GNPIIVYLHG SAEHRAASHR LKLVKVLSDG
61GFHVLSVDYR GFGDSTGKPT EEGLTTDAIC VYEWTKARSG ITPVCLWGHS LGTGVATNAA
121KVLEEKGCPV DAIVLEAPFT NMWVASINYP LLKIYRNIPG FLRTLMDALR KDKIIFPNDE
181NVKFLSSPLL ILHGEDDRTV PLEYGKKLYE IARNAYRNKE RVKMVIFPPG FQHNLLCKSP
241TLLITVRDFL SKQWS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

255 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.