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ABCF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCF3 (NM_018358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABCF3 | EST201864
Origin Species
Human
NCBI GenBank
NM_018358
SwissProt
Q9NUQ8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATCAEILRS EFPEIDGQVF DYVTGVLHSG SADFESVDDL VEAVGELLQE VSGDSKDDAG
61IRAVCQRMYN TLRLAEPQSQ GNSQVLLDAP IQLSKITENY DCGTKLPGLL KREQSSTVNA
121KKLEKAEARL KAKQEKRSEK DTLKTSNPLV LEEASASQAG SRKESRLESS GKNKSYDVRI
181ENFDVSFGDR VLLAGADVNL AWGRRYGLVG RNGLGKTTLL KMLATRSLRV PAHISLLHVE
241QEVAGDDTPA LQSVLESDSV REDLLRRERE LTAQIAAGRA EGSEAAELAE IYAKLEEIEA
301DKAPARASVI LAGLGFTPKM QQQPTREFSG GWRMRLALAR ALFARPDLLL LDEPTNMLDV
361RAILWLENYL QTWPSTILVV SHDRNFLNAI ATDIIHLHSQ RLDGYRGDFE TFIKSKQERL
421LNQQREYEAQ QQYRQHIQVF IDRFRYNANR ASQVQSKLKM LEKLPELKPV DKESEVVMKF
481PDGFEKFSPP ILQLDEVDFY YDPKHVIFSR LSVSADLESR ICVVGENGAG KSTMLKLLLG
541DLAPVRGIRH AHRNLKIGYF SQHHVEQLDL NVSAVELLAR KFPGRPEEEY RHQLGRYGIS
601GELAMRPLAS LSGGQKSRVA FAQMTMPCPN FYILDEPTNH LDMETIEALG RALNNFRGGV
661ILVSHDERFI RLVCRELWVC EGGGVTRVEG GFDQYRALLQ EQFRREGFLT RTRPLEQKLI
721SEEDLAANDI LDYKDDDDKV

740 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.