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ABCF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100984-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCF2 (NM_007189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABC-type transport protein | ABC28 | ABCF2 | EST133090 | M-ABC1 | HUSSY18 | HUSSY-18
Origin Species
Human
NCBI GenBank
NM_005692
SwissProt
Q9UG63

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSDLAKKKA AKKKEAAKAR QRPRKGHEEN GDVVTEPQVA EKNEANGRET TEVDLLTKEL
61EDFEMKKAAA RAVTGVLASH PNSTDVHIIN LSLTFHGQEL LSDTKLELNS GRRYGLIGLN
121GIGKSMLLSA IGKREVPIPE HIDIYHLTRE MPPSDKTPLH CVMEVDTERA MLEKEAERLA
181HEDAECEKLM ELYERLEELD ADKAEMRASR ILHGLGFTPA MQRKKLKDFS GGWRMRVALA
241RALFIRPFML LLDEPTNHLD LDACVWLEEE LKTFKRILVL VSHSQDFLNG VCTNIIHMHN
301KKLKYYTGNY DQYVKTRLEL EENQMKRFHW EQDQIAHMKN YIARFGHGSA KLARQAQSKE
361KTLQKMMASG LTERVVSDKT LSFYFPPCGK IPPPVIMVQN VSFKYTKDGP CIYNNLEFGI
421DLDTRVALVG PNGAGKSTLL KLLTGELLPT DGMIRKHSHV KIGRYHQHLQ EQLDLDLSPL
481EYMMKCYPEI KEKEEMRKII GRYGLTGKQQ VSPIRNLSDG QKCRVCLAWL AWQNPHMLFL
541DEPTNHLDIE TIDALADAIN EFEGGMMLVS HDFRLIQQVA QEIWVCEKQT ITKWPGDILA
601YKEHLKSKLV DEEPQLTKRT HNVTRTRPLE QKLISEEDLA ANDILDYKDD DDKV

654 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.