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ABCB7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCB7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ABCB7 antibodies →
$504.00each
Catalog Number: LS-G116747-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCB7 (NM_004299), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ABC transporter 7 protein | ABC7 | ABCB7 | Atm1p | ATP-binding cassette 7 | ASAT | EST140535
Origin Species
Human
NCBI GenBank
NM_004299
SwissProt
O75027

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MALLAMHSWR WAAAAAAFEK RRHSAILIRP LVSVSGSGPQ WRPHQLGALG TARAYQQIPE
61SLKSITWQRL GKGNSGQFLD AAKALQVWPL IEKRTCWHGH AGGGLHTDPK EGLKDVDTRK
121IIKAMLSYVW PKDRPDLRAR VAISLGFLGG AKAMNIVVPF MFKYAVDSLN QMSGNMLNLS
181DAPNTVATMA TAVLIGYGVS RAGAAFFNEV RNAVFGKVAQ NSIRRIAKNV FLHLHNLDLG
241FHLSRQTGAL SKAIDRGTRG ISFVLSALVF NLLPIMFEVM LVSGVLYYKC GAQFALVTLG
301TLGTYTAFTV AVTRWRTRFR IEMNKADNDA GNAAIDSLLN YETVKYFNNE RYEAQRYDGF
361LKTYETASLK STSTLAMLNF GQSAIFSVGL TAIMVLASQG IVAGTLTVGD LVMVNGLLFQ
421LSLPLNFLGT VYRETRQALI DMNTLFTLLK VDTQIKDKVM ASPLQITPQT ATVAFDNVHF
481EYIEGQKVLS GISFEVPAGK KVAIVGGSGS GKSTIVRLLF RFYEPQKGSI YLAGQNIQDV
541SLESLRRAVG VVPQDAVLFH NTIYYNLLYG NISASPEEVY AVAKLAGLHD AILRMPHGYD
601TQVGERGLKL SGGEKQRVAI ARAILKDPPV ILYDEATSSL DSITEETILG AMKDVVKHRT
661SIFIAHRLST VVDADEIIVL DQGKVAERGT HHGLLANPHS IYSEMWHTQS SRVQNHDNPK
721WEAKKENISK EEERKKLQEE IVNSVKGCGN CSC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

753 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.