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AARSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AARSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109515-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AARSD1(PTGES3L-AARSD1) (NM_025267), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AARSD1
Origin Species
Human
SwissProt
Q9BTE6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEFCVEDSTD VHVLIEDHRI VFSCKNADGV ELYNEIEFYA KVNSKDSQDK RSSRSITCFV
61RKWKEKVAWP RLTKEDIKPV WLSVDFDNWR DWEGDEEMEL AHVEHYAELL KKVSTKRPPP
121AMDDLDFTTT VVSCCPAELQ TEGSNGKKEV LSGFQVVLED TVLFPEGGGQ PDDRGTINDI
181SVLRVTRRGE QADHFTQTPL DPGSQVLVRV DWERRFDHMQ QHSGQHLITA VADHLFKLKT
241TSWELGRFRS AIELDTPSMT AEQVAAIEQS VNEKIRDRLP VNVRELSLDD PEVEQVSGRG
301LPDDHAGPIR VVNIEGVDSN MCCGTHVSNL SDLQVIKILG TEKGKKNRTN LIFLSGNRVL
361KWMERSHGTE KALTALLKCG AEDHVEAVKK LQNSTKILQK NNLNLLRDLA VHIAHSLRNS
421PDWGGVVILH RKEGDSEFMN IIANEIGSEE TLLFLTVGDE KGGGLFLLAG PPASVETLGP
481RVAEVLEGKG AGKKGRFQGK ATKMSRRMEA QALLQDYIST QSAKETRTRP LEQKLISEED
541LAANDILDYK DDDDKV

556 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.