Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
A1CF / ACF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113862-100
Catalog Number: LS-G113862
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human A1CF (NM_014576), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A1CF | ACF65 | ACF64 | APOBEC-1 stimulating protein | APOBEC1 complementation factor | APOBEC1CF | ACF | Apo-B RNA editing protein | APOBEC1-stimulating protein | RP11-564C4.2 | ASP
- Origin Species
- Human
- NCBI GenBank
- NM_014576
- SwissProt
- Q9NQ94
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESNHKSGDG LSGTQKEAAL RALVQRTGYS LVQENGQRKY GGPPPGWDAA PPERGCEIFI61GKLPRDLFED ELIPLCEKIG KIYEMRMMMD FNGNNRGYAF VTFSNKVEAK NAIKQLNNYE121IRNGRLLGVC ASVDNCRLFV GGIPKTKKRE EILSEMKKVT EGVVDVIVYP SAADKTKNRG181FAFVEYESHR AAAMARRKLL PGRIQLWGHG IAVDWAEPEV EVDEDTMSSV KILYVRNLML241STSEEMIEKE FNNIKPGAVE RVKKIRDYAF VHFSNREDAV EAMKALNGKV LDGSPIEVTL301AKPVDKDSYV RYTRGTGGRG TMLQGEYTYS LGQVYDPTTT YLGAPVFYAP QTYAAIPSLH361FPATKGHLSN RAIIRAPSVR GAAGVRGLGG RGYLAYTGLG RGYQVKGDKR EDKLYDILPG421MELTPMNPVT LKPQGIKLAP QILEEICQKN NWGQPVYQLH SAIGQDQRQL FLYKITIPAL481ASQNPAIHPF TPPKLSAFVD EAKTYAAEYT LQTLGIPTDG GDGTMATAAA AATAFPGYAV541PNATAPVSAA QLKQAVTLGQ DLAAYTTYEV YPTFAVTARG DGYGTFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
586 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.