LSBio
Rat NOX4 ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Rat NOX4 ELISA Kit (Sandwich ELISA)

Reactivity:Rat
Detection MethodColorimetric
Format:96-Well Strip Plate
See all NOX4 antibodies →
$765.00each
Catalog Number: LS-F5720-1

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Overview

Description
NOX4 ELISA Kit LS-F5720 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Rat NOX4 in Cell Lysates, Tissue Homogenates. It is a Sandwich assay which can detect NOX4 down to 0.312 ng/ml. For Research Use Only
Reactivity
Rt
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
Kidney oxidase-1 | KOX | RENOX | KOX-1 | NADPH oxidase 4 | NOX4 | Renal NAD(P)H-oxidase

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
0.113 ng/ml
Detection Range
0.312 - 20 ng/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Lysate, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Activated ROCK/Akt/eNOS and ET-1/ERK pathways in 5-fluorouracil-induced cardiotoxicity: modulation by simvastatin. Radwa Nasser Muhammad, Nada Sallam, Hanan Salah El-Abhar. Scientific reports. 2020 September;10:14693.

PubMed: 32895407 · PMC: PMC7477553


In Vivo Investigation of the Ameliorating Effect of Tempol against MIA-Induced Knee Osteoarthritis in Rats: Involvement of TGF-ß1/SMAD3/NOX4 Cue. Hagar B Abo-Zalam, Rania M Abdelsalam , Rehab F Abdel-Rahman, Mohamed F Abd-Ellah, Mahmoud M Khattab. Molecules (Basel, Switzerland). 2021 Nov;26:6993.

PubMed: 34834085 · PMC: PMC8618489

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.