LSBio
Rat HMOX1 / HO-1 ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Rat HMOX1 / HO-1 ELISA Kit (Sandwich ELISA)

Reactivity:Rat
Detection MethodColorimetric
Format:96-Well Strip Plate
See all HMOX1 / HO-1 antibodies →
$730.00each
Catalog Number: LS-F4085-1

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Overview

Description
HMOX1 / HO-1 ELISA Kit LS-F4085 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Rat HMOX1 / HO-1 in Cell Culture Supernatants, Cell Lysates, Tissue Homogenates. It is a Sandwich assay which can detect HMOX1 / HO-1 down to 0.312 ng/ml. For Research Use Only
Reactivity
Rt
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
BK286B10 | HO-1 | HSP32 | HMOX1 | Heat shock protein, 32-kD | Heme oxygenase (decycling) 1 | Heme oxygenase 1 | HO1

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
0.121 ng/ml
Detection Range
0.312 - 20 ng/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Resuscitation with PEGylated carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia. Philippe Guerci, Bülent Ergin, Asli Kandil, Yasin Ince, Paul Heeman, Matthias Peter Hilty, Jan Bakker, Can Ince. American journal of physiology. Renal physiology. 2020 May;318:F1271-F1283.

PubMed: 32281418


Effect of Polyethylene-glycolated Carboxyhemoglobin on Renal Microcirculation in a Rat Model of Hemorrhagic Shock. Philippe Guerci, Bulent Ergin, Aysegul Kapucu, Matthias P Hilty, Ronald Jubin, Jan Bakker, Can Ince. Anesthesiology. 2019 November;131:1110-1124.

PubMed: 31490291

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