LSBio
Rat Collagen I ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Rat Collagen I ELISA Kit (Sandwich ELISA)

ELISA KitLSBio Guarantee
Reactivity:Rat
Detection MethodColorimetric
Format:96-Well Strip Plate
See all Collagen I antibodies →
$730.00each
Catalog Number: LS-F5638-1

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Overview

Description
Collagen I ELISA Kit LS-F5638 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Rat Collagen I in Plasma, Serum. It is a Sandwich assay which can detect Collagen I down to 0.625 ng/ml. For Research Use Only
Reactivity
Rt
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
0.219 ng/ml
Detection Range
0.625 - 40 ng/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Plasma, Serum
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Substance P increases CCN2 dependent on TGF-beta yet Collagen Type I via TGF-beta1 dependent and independent pathways in tenocytes. Frara N, Fisher PW, Zhao Y, Tarr JT, Amin M, Popoff SN, Barbe MF. Connective tissue research. 2018 January;59:30-44.

PubMed: 28399671 · PMC: PMC5581284

Comparing effects of rest with or without a NK1RA on fibrosis and sensorimotor declines induced by a voluntary moderate demand task. Barbe MF, White AR, Hilliard BA, Salvadeo DM, Amin M, Harris MY, Cruz GE, Hobson L, Popoff SN. Journal of Musculoskeletal and Neuronal Interactions. 2019 December;19:396-411.

PubMed: 31789291 · PMC: PMC6944794

Key indicators of repetitive overuse-induced neuromuscular inflammation and fibrosis are prevented by manual therapy in a rat model. Mary F Barbe, Michele Y Harris, Geneva E Cruz, Mamta Amin, Nathan M Billett, Jocelynne T Dorotan, Emily P Day, Seung Y Kim, Geoffrey M Bove. BMC musculoskeletal disorders. 2021 May;22:417.

PubMed: 33952219 · PMC: PMC8101118

Blocking substance P signaling reduces musculotendinous and dermal fibrosis and sensorimotor declines in a rat model of overuse injury. M F Barbe, B A Hilliard, P W Fisher, A R White, S P Delany, V J Iannarone, M Y Harris, M Amin, G E Cruz, S N Popoff. Connective tissue research. 2019 Nov;61:604-619.

PubMed: 31443618 · PMC: PMC7036028

Manual Therapy With Rest as a Treatment for Established Inflammation and Fibrosis in a Rat Model of Repetitive Strain Injury. Mary F Barbe, Siva Tejaa Panibatla, Michele Y Harris, Mamta Amin, Jocelynne T Dorotan, Geneva E Cruz, Geoffrey M Bove. Frontiers in physiology. 2021 Nov;12:755923.

PubMed: 34803739 · PMC: PMC8600143

Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles. Brendan A Hilliard, Mamta Amin, Steven N Popoff, Mary F Barbe. Connective tissue research. 2021 January;62:133-149.

PubMed: 33030055 · PMC: PMC7718395

Long-Term Dipeptidyl Peptidase 4 Inhibition Worsens Hypertension and Renal and Cardiac Abnormalities in Obese Spontaneously Hypertensive Heart Failure Rats. Edwin K Jackson, Zaichuan Mi, Delbert G Gillespie, Dongmei Cheng, Stevan P Tofovic. Journal of the American Heart Association. 2021 March;10:e020088.

PubMed: 33682436 · PMC: PMC8174220

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