LSBio
Mouse Fgf15 ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Mouse Fgf15 ELISA Kit (Sandwich ELISA)

Reactivity:Mouse
Detection MethodColorimetric
Format:96-Well Strip Plate
See all Fgf15 antibodies →
$699.00each
Catalog Number: LS-F11446-1

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Overview

Description
Fgf15 ELISA Kit LS-F11446 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Mouse Fgf15 in Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect Fgf15 down to 78 pg/ml. For Research Use Only
Reactivity
Ms
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
39.4 pg/ml
Detection Range
78 - 5000 pg/ml
Intra-Assay CV%
<4.9%
Inter-Assay CV%
<7.7%
Compatible Sample Types
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Adhesive Plate Sealers, Wash Buffer (25x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Myeloid Cell-Specific Lipin-1 Deficiency Stimulates Endocrine Adiponectin-FGF15 Axis and Ameliorates Ethanol-Induced Liver Injury in Mice. Wang J, Kim C, Jogasuria A, Han Y, Hu X, Wu J, Shen H, Chrast R, Finck BN, You M. Scientific reports. 2016 September;6:34117.

PubMed: 27666676 · PMC: PMC5036185


Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Huang F, Zheng X, Ma X, Jiang R, Zhou W, Zhou S, Zhang Y, Lei S, Wang S, Kuang J, Han X, Wei M, You Y, Li M, Li Y, Liang D, Liu J, Chen T, Yan C, Wei R, Rajani C, Shen C, Xie G, Bian Z, Li H, Zhao A, Jia W. Nature communications. 2019 October;10:4971.

PubMed: 31672964 · PMC: PMC6823360


Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease. Michael D Thompson, Alaina Derse, Jeremie LA Ferey, Michaela Reid, Yan Xie, Miranda Christ, Deyali Chatterjee, Chau Nguyen, Natalia Harasymowicz, Farshid Guilak, Kelle H Moley, Nicholas Oliver Davidson. American journal of physiology. Endocrinology and metabolism. 2019 April;316:E674-E686.

PubMed: 30860882 · PMC: PMC6482665


Selective PPARd agonist seladelpar suppresses bile acid synthesis by reducing hepatocyte CYP7A1 via the fibroblast growth factor 21 signaling pathway. Tetsuya Kouno, Xiao Liu, Huayi Zhao, Tatiana Kisseleva, Edward E Cable, Bernd Schnabl. The Journal of biological chemistry. 2022 July;298:102056.

PubMed: 35605662 · PMC: PMC9214809


FGF15 promotes hepatic NPC1L1 degradation in lithogenic diet-fed mice. Pingfan Mo , Hongtan Chen , Xin Jiang, Fengling Hu, Fenming Zhang, Guodong Shan, Wenguo Chen, Sha Li, Yiqiao Li, Guoqiang Xu. Lipids in health and disease. 2022 October;21:97.

PubMed: 36209166 · PMC: PMC9547418

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.